Neutrophlis extracelullar traps (NETS) on the genesis and development of periapical lesion on Wild-type and Knockout animals

• Grant number: 20/05797-8
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2020
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Raquel Assed Bezerra da Silva
• Grantee: Raquel Assed Bezerra da Silva
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Carolina Maschietto Pucinelli ; Léa Assed Bezerra da Silva

Abstract

Following fifteen years of research, Neutrophil Extracellular Traps (NETs) are widely reported in a large range of inflammatory infectious and non-infectious diseases. Cumulating evidences from in vitro, in vivo and clinical suggest that NETs may play a crucial role in inflammation and autoimmunity in a variety of autoimmune diseases, such as rheumatoid arthritis, lupus erythematosus, and others. Indeed, NETs have not only been implicated as drivers of inflammation, but also are linked to resolution of inflammation. Therefore, serve as biomarkers, as well as promising targets for future therapeutics of inflammatory autoimmune diseases. The aim of the present study will be evaluate the role of NETs, in the presence of PAD4 (peptidyl arginine deiminase-4) on the development of periapical lesions in wild-type e knockout animals. 55 animals will be used, including PAD4 knockout mice and wild-type (WT) animals. Induction of periapical lesion will be performed in all animals of all groups in the experimental periods of 2, 14 and 42 days and will be performed according to the method already used in previous studies of our research group (Silva et al., 2012; Bezerra da Silva et al., 2014; Oliveira et al., 2015; De Rossi et al., 2016; Barreiros et al., 2018). The genesis and development of periapical lesions will be evaluated by of microscopic analysis with hematoxylin and eosin (HE) staining (descriptive analysis); computed micro-tomography (measurement of periapical lesions); RT-PCR for reactive oxygen species (ROS), myeloperoxidase, elastase and cathepsin-G; and immunohistochemistry for ROS, myeloperoxidase, elastase and cathepsin-G. The numerical data obtained will be analyzed using the Graph Pad Prism 7 statistical program (Graph Pad Software In., San Diego, California, USA), using the appropriate tests for each evaluation. The level of significance adopted will be 5%. (AU)