EMU approved in grant 15 / 50590-4: chromatographic system and detectors for analysis of sugars and lignocellulosic monolignols

• Grant number: 20/05784-3
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2020
• End date: October 31, 2027
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Fábio Márcio Squina
• Grantee: Fábio Márcio Squina
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals, AP.BIOEN.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The sustainable production of chemicals and biofuels from lignocellulosic biomass is an alternative to mitigating the emission of greenhouse gases and dependence on oil industries. Besides, the transition from a fossil fuel-based economy to a renewable bioeconomy should promote job creation and economic growth. Biotechnologies for the valorization of lignocelluloses are a great challenge, as they need to be competitive with the current processes of petrochemical refineries. In this context, a facility laboratory with an open infrastructure for support biotechnological researches is urgently required. The aim of this multi-user equipment (EMU) is to carry out analyses of saccharides (native and oxidized) and derivatives of hydroxybenzoic and hydroxycinnamic acids, that will accelerate the scientific advance of research groups working in the field of converting lignocellulose into chemical bioproducts. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, THIAGO AUGUSTO; SODRE, VICTORIA; DA SILVA, STEPHANIE NEMESIO; VILELA, NATHALIA; TOMAZETTO, GEIZECLER; ARAUJO, JUSCEMACIA NASCIMENTO; MUNIZ, JOAO RENATO C.; FILL, TAICIA PACHECO; DAMASIO, ANDRE; GARCIA, WANIUS; et al. Applying biochemical and structural characterization of hydroxycinnamate catabolic enzymes from soil metagenome for lignin valorization strategies. Applied Microbiology and Biotechnology, v. 106, n. 7, p. 14-pg., 2022-03-30. (17/16976-8, 20/05784-3, 18/18101-1, 17/17275-3, 17/22669-0, 15/50612-8, 15/50590-4, 15/23279-6)

LIBERATO, MARCELO VIZONA; CAMPOS, BRUNA MEDEIA; TOMAZETTO, GEIZECLER; CROUCH, LUCY ISOBEL; GARCIA, WANIUS; DE MATTOS ZERI, ANA CAROLINA; BOLAM, DAVID NICHOL; SQUINA, FABIO MARCIO.

Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions

. Journal of Biological Chemistry, v. 298, n. 5, p. 10-pg., 2022-05-02. (15/50590-4, 13/06336-0, 15/23279-6, 20/15595-3, 14/04105-4, 20/05784-3, 15/14009-5, 17/17275-3)

PIMENTEL, AGNES C.; LIBERATO, MARCELO V.; CAIRO, JOAO PAULO L. FRANCO; TOMAZETTO, GEIZECLER; GANDIN, CESAR A.; NETO, MARIO DE OLIVEIRA; ALVAREZ, THABATA M.; SQUINA, FABIO M.. Structural and functional insights of the catalytic GH5 and Calx-beta domains from the metagenome-derived endoglucanase CelE2. Enzyme and Microbial Technology, v. 165, p. 10-pg., 2023-04-01. (16/01926-2, 15/50590-4, 20/05784-3, 14/04105-4, 10/11469-1, 16/09950-0, 15/23279-6)

DE FIGUEIREDO, FERNANDA LOPES; DE OLIVEIRA, ANA CAROLINA PIVA; TERRASAN, CESAR RAFAEL FANCHINI; GONCALVES, THIAGO AUGUSTO; GERHARDT, JAQUELINE ALINE; TOMAZETTO, GEIZECLER; PERSINOTI, GABRIELA FELIX; RUBIO, MARCELO VENTURA; PENA, JENNIFER ANDREA TAMAYO; ARAUJO, MICHELLE FERNANDES; et al. Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600. BIOTECHNOLOGY FOR BIOFUELS, v. 14, n. 1, APR 17 2021. (19/21609-0, 15/50612-8, 17/22669-0, 20/05784-3, 19/12860-0, 15/50590-4)

BRENELLI, LIVIA BEATRIZ; MARIUTTI, LILIAN REGINA BARROS; PORTUGAL, RODRIGO VILLARES; DE FARIAS, MARCELO ALEXANDRE; BRAGAGNOLO, NEURA; MERCADANTE, ADRIANA ZERLOTTI; FRANCO, TELMA TEIXEIRA; RABELO, SARITA CANDIDA; SQUINA, FABIO MARCIO. odified lignin from sugarcane bagasse as an emulsifier in oil-in-water nanoemulsion. INDUSTRIAL CROPS AND PRODUCTS, v. 167, SEP 1 2021. (13/03061-0, 20/05784-3, 15/50612-8, 15/50590-4)

DOS SANTOS, OTAVIO AUGUSTO LEITAO; GONCALVES, THIAGO AUGUSTO; SODRE, VICTORIA; VILELA, NATHALIA; TOMAZETTO, GEIZECLER; SQUINA, FABIO M.; GARCIA, WANIUS. Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production. Protein Expression and Purification, v. 197, p. 7-pg., 2022-05-11. (17/16089-1, 15/50590-4, 18/18101-1, 17/08166-6, 20/15595-3, 20/05784-3, 17/17275-3)

ANDRADE, VIVIANE BRITO; TOMAZETTO, GEIZECLER; ALMEIDA, DNANE VIEIRA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; GARCIA, WANIUS. Enzymatic and biophysical characterization of a novel modular cellulosomal GH5 endoglucanase multifunctional from the anaerobic gut fungus Piromyces finnis. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1872, n. 1, p. 11-pg., 2024-01-01. (20/15595-3, 20/05784-3, 21/04254-3, 15/23279-6, 22/08958-8, 15/50590-4)