EMU approved in grant 15 / 50590-4: chromatographic system and detectors for analysis of sugars and lignocellulosic monolignols

• Grant number: 20/05784-3
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2020
• End date: October 31, 2027
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Fábio Márcio Squina
• Grantee: Fábio Márcio Squina
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated research grant: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals, AP.BIOEN.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The sustainable production of chemicals and biofuels from lignocellulosic biomass is an alternative to mitigating the emission of greenhouse gases and dependence on oil industries. Besides, the transition from a fossil fuel-based economy to a renewable bioeconomy should promote job creation and economic growth. Biotechnologies for the valorization of lignocelluloses are a great challenge, as they need to be competitive with the current processes of petrochemical refineries. In this context, a facility laboratory with an open infrastructure for support biotechnological researches is urgently required. The aim of this multi-user equipment (EMU) is to carry out analyses of saccharides (native and oxidized) and derivatives of hydroxybenzoic and hydroxycinnamic acids, that will accelerate the scientific advance of research groups working in the field of converting lignocellulose into chemical bioproducts. (AU)

Scientific publications

(14)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

DE FIGUEIREDO, FERNANDA LOPES; DE OLIVEIRA, ANA CAROLINA PIVA; TERRASAN, CESAR RAFAEL FANCHINI; GONCALVES, THIAGO AUGUSTO; GERHARDT, JAQUELINE ALINE; TOMAZETTO, GEIZECLER; PERSINOTI, GABRIELA FELIX; RUBIO, MARCELO VENTURA; PENA, JENNIFER ANDREA TAMAYO; ARAUJO, MICHELLE FERNANDES; et al. Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600. BIOTECHNOLOGY FOR BIOFUELS, v. 14, n. 1, APR 17 2021. (19/21609-0, 15/50612-8, 17/22669-0, 20/05784-3, 19/12860-0, 15/50590-4)

BRENELLI, LIVIA BEATRIZ; MARIUTTI, LILIAN REGINA BARROS; PORTUGAL, RODRIGO VILLARES; DE FARIAS, MARCELO ALEXANDRE; BRAGAGNOLO, NEURA; MERCADANTE, ADRIANA ZERLOTTI; FRANCO, TELMA TEIXEIRA; RABELO, SARITA CANDIDA; SQUINA, FABIO MARCIO. odified lignin from sugarcane bagasse as an emulsifier in oil-in-water nanoemulsion. INDUSTRIAL CROPS AND PRODUCTS, v. 167, SEP 1 2021. (13/03061-0, 20/05784-3, 15/50612-8, 15/50590-4)

DOS SANTOS, OTAVIO AUGUSTO LEITAO; GONCALVES, THIAGO AUGUSTO; SODRE, VICTORIA; VILELA, NATHALIA; TOMAZETTO, GEIZECLER; SQUINA, FABIO M.; GARCIA, WANIUS. Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production. Protein Expression and Purification, v. 197, p. 7-pg., 2022-05-11. (17/16089-1, 15/50590-4, 18/18101-1, 17/08166-6, 20/15595-3, 20/05784-3, 17/17275-3)

PIMENTEL, AGNES C.; LIBERATO, MARCELO V.; CAIRO, JOAO PAULO L. FRANCO; TOMAZETTO, GEIZECLER; GANDIN, CESAR A.; NETO, MARIO DE OLIVEIRA; ALVAREZ, THABATA M.; SQUINA, FABIO M.. Structural and functional insights of the catalytic GH5 and Calx-beta domains from the metagenome-derived endoglucanase CelE2. Enzyme and Microbial Technology, v. 165, p. 10-pg., 2023-04-01. (16/01926-2, 15/50590-4, 20/05784-3, 14/04105-4, 10/11469-1, 16/09950-0, 15/23279-6)

ANDRADE, VIVIANE BRITO; TOMAZETTO, GEIZECLER; ALMEIDA, DNANE VIEIRA; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; GARCIA, WANIUS. Enzymatic and biophysical characterization of a novel modular cellulosomal GH5 endoglucanase multifunctional from the anaerobic gut fungus Piromyces finnis. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1872, n. 1, p. 11-pg., 2024-01-01. (20/15595-3, 20/05784-3, 21/04254-3, 15/23279-6, 22/08958-8, 15/50590-4)

SANTANA JR, MARCEL B.; GAMA, FELIPE AVILA; PEREIRA, ISABELA O.; TRAMONTINA, ROBSON; SQUINA, FABIO MARCIO; AMBROSI, ALAN; ZIELINSKI, ACACIO; POLETTO, PATRICIA; IENCZAK, JACIANE L.. Harnessing tobacco stem biomass for eco-friendly xylo-oligomers production via hydrothermal treatment and succinic acid via fermentation. JOURNAL OF CLEANER PRODUCTION, v. 456, p. 12-pg., 2024-05-02. (20/05784-3, 22/08958-8, 21/04254-3)

ROMAN, ELLEN KAREN BARRETO; RAMOS, MURILO ANTONIO; TOMAZETTO, GEIZECLER; FOLTRAN, BRUNO BOTEGA; GALVAO, MATHEUS HENRIQUE; CIANCAGLINI, IARA; TRAMONTINA, ROBSON; RODRIGUES, FELIPE DE ALMEIDA; DA SILVA, LARISSA SOARES; SANDANO, ANA LUIZA HERNANDES; et al. Plastic-degrading microbial communities reveal novel microorganisms, pathways, and biocatalysts for polymer degradation and bioplastic production. Science of The Total Environment, v. 949, p. 14-pg., 2024-08-02. (19/19360-3, 23/04941-6, 20/05784-3, 23/04828-5, 15/23279-6, 23/08832-7, 20/11019-8, 23/04782-5, 21/04254-3, 22/08958-8, 23/12398-0, 22/05731-2, 22/14112-4)

PROCOPIO, DIELLE PIEROTTI; LEE, JAE WON; SHIN, JONGHYEOK; TRAMONTINA, ROBSON; AVILA, PATRICIA FELIX; BRENELLI, LIVIA BEATRIZ; SQUINA, FABIO MARCIO; DAMASIO, ANDRE; RABELO, SARITA CANDIDA; GOLDBECK, ROSANA; et al. Metabolic engineering of Saccharomyces cerevisiae for second-generation ethanol production from xylo-oligosaccharides and acetate. SCIENTIFIC REPORTS, v. 13, n. 1, p. 14-pg., 2023-11-06. (17/15477-8, 20/05784-3, 15/50612-8, 18/17172-2, 18/01759-4, 22/08958-8, 21/04254-3, 15/50590-4, 22/05731-2, 19/18075-3)

LIBERATO, MARCELO VIZONA; PAIXAO, DOUGLAS ANTONIO ALVAREDO; TOMAZETTO, GEIZECLER; NDEH, DIDIER; BOLAM, DAVID N.; SQUINA, FABIO MARCIO. Discovery, structural characterization, and functional insights into a novel apiosidase from the GH140 family, isolated from a lignocellulolytic-enriched mangrove microbial community. Biotechnology Letters, v. 46, n. 2, p. 11-pg., 2024-01-27. (17/12597-2, 14/04105-4, 20/05784-3, 22/08958-8)

VILELA, NATHALIA; GORKOVSKIY, ANTON; HERRERA-MALAVER, BEATRIZ; TRAMONTINA, ROBSON; CAIRO, JOAO PAULO L. FRANCO; MANDELLI, FERNANDA; GONCALVES, THIAGO; SODRE, VICTORIA; STEENSELS, JAN; DAMASIO, ANDRE; et al. From residues to clove: Harnessing novel phenolic acid decarboxylase for 4-Vinyl guaiacol production in industrial Yeast. FOOD AND BIOPRODUCTS PROCESSING, v. 151, p. 8-pg., 2025-03-06. (20/05784-3, 22/05731-2, 22/08958-8, 15/50590-4, 20/10593-2, 17/08166-6, 21/04254-3)

GONCALVES, THIAGO AUGUSTO; SODRE, VICTORIA; DA SILVA, STEPHANIE NEMESIO; VILELA, NATHALIA; TOMAZETTO, GEIZECLER; ARAUJO, JUSCEMACIA NASCIMENTO; MUNIZ, JOAO RENATO C.; FILL, TAICIA PACHECO; DAMASIO, ANDRE; GARCIA, WANIUS; et al. Applying biochemical and structural characterization of hydroxycinnamate catabolic enzymes from soil metagenome for lignin valorization strategies. Applied Microbiology and Biotechnology, v. 106, n. 7, p. 14-pg., 2022-03-30. (17/16976-8, 20/05784-3, 18/18101-1, 17/17275-3, 17/22669-0, 15/50612-8, 15/50590-4, 15/23279-6)

TRAMONTINA, ROBSON; SCOPEL, EUPIDIO; BRENELLI, LIVIA; NOGUEIRA, GUILHERME P.; FRANCO, TELMA T.; REZENDE, CAMILA A.; GOLDBECK, ROSANA; SQUINA, FABIO M.. Applying biorefinery concepts for sugarcane straw upcycling using alkaline and enzymatic treatments to produce value-added compounds and bioenergy. BIOMASS & BIOENERGY, v. 178, p. 9-pg., 2023-10-12. (19/19360-3, 17/15477-8, 15/50612-8, 20/05784-3, 18/23769-1, 22/08958-8, 21/04254-3, 15/50590-4)

LIBERATO, MARCELO VIZONA; CAMPOS, BRUNA MEDEIA; TOMAZETTO, GEIZECLER; CROUCH, LUCY ISOBEL; GARCIA, WANIUS; DE MATTOS ZERI, ANA CAROLINA; BOLAM, DAVID NICHOL; SQUINA, FABIO MARCIO.

Unique properties of a Dictyostelium discoideum carbohydrate-binding module expand our understanding of CBM-ligand interactions

. Journal of Biological Chemistry, v. 298, n. 5, p. 10-pg., 2022-05-02. (15/50590-4, 13/06336-0, 15/23279-6, 20/15595-3, 14/04105-4, 20/05784-3, 15/14009-5, 17/17275-3)

CAIRO, JOA O PAULO L. FRANCO; ALMEIDA, DNANE V.; ANDRADE, VIVIANE B.; TELFER, ABBEY; GONCALVES, THIAGO A.; DIAZ, DANIEL E.; FIGUEIREDO, FERNANDA L.; BRENELLI, LIVIA B.; WALTON, PAUL H.; GARCIA, WANIUS; et al. Biochemical and structural insights of a recombinant AA16 LPMO from the marine and sponge-symbiont Peniophora sp. International Journal of Biological Macromolecules, v. 280, p. 11-pg., 2024-09-18. (20/05784-3, 22/08958-8, 18/13500-5, 15/50590-4, 22/05731-2, 17/17275-3)