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The effect of L-leucine supplementation associated with sprint interval training on caloric intake and appetite control markers in humans

Grant number: 20/09936-2
Support type:Regular Research Grants
Duration: April 01, 2021 - March 31, 2023
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal researcher:Érico Chagas Caperuto
Grantee:Érico Chagas Caperuto
Home Institution: Faculdade de Ciências Biológicas e da Saúde. Universidade São Judas Tadeu (USJT). São Paulo , SP, Brazil
Assoc. researchers: Adam R Jajtner ; Dr Peter Hofmann ; Elias de França ; RAUL AGOSTINHO SIMÕES MARTINS ; Rita de Cássia de Aquino ; Ronaldo Vagner Thomatieli dos Santos


High-intensity exercise may be a tool for appetite control and a treatment for obesity, through an acute inflammatory response, improving insulin sensitivity, increasing lactate concentrations and appetite-modulating neuropeptides. L-leucine supplementation modulates appetite activating the mTORC pathway, inducing increased appetite- modifying neuropeptides and increasing insulin sensitivity. The aim of this study is to evaluate the association of L-leu supplementation and the sprint interval training (SIT) on appetite control in humans. The study will be double-blind, randomized, counterbalanced and crossover. Participants will perform four experiments: 1) SIT plus leucine supplementation, 2) SIT plus placebo supplementation, 3) leucine supplementation without exercise and 4) placebo supplementation without exercise. Supplementation will consist of three daily doses of 70 mg/kg of body weight of leucine or placebo (in the SIT day and one day after). One day prior to SIT and L-leu supplementation the volunteers will record the nutrient intake. Immediately before, immediately, 30 and 60 min after the SIT, appetite perception and blood sample will be collected for GLP-1, PYY, CCK, ghrelin, IL-6, IL-10 and TNF-± analysis. The data will be analyzed using a repeated measure analysis of variance (ANOVA) test with a Bonferroni post-test. Our pilot study shows appetite perception decrease in fasting state, before SIT (30 minutes before breakfast), immediately and 30 minutes after SIT. Supplementation avoids caloric ingestion compensation presented in the placebo group. Together these data suggest that supplementation associated with exercise promotes appetite changes. (AU)