Evaluating the nanotoxicity using image-based multiparametric single-cell profiling

• Grant number: 20/01218-3
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2021
• End date: June 30, 2023
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Marcelo Bispo de Jesus
• Grantee: Marcelo Bispo de Jesus
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Nanomaterials have been used in the everyday life, across products for home, and medical applications. The wide spread of nanomaterials in the environment have caused the accumulation in water and biosolids at low concentrations, which can cause human intoxication and alterations from a lifetime exposure. However, nanotoxic studies have focused on cell death caused by high concentrations. Our project aims to investigate subtle alterations triggered by exposure to nanoparticles at low concentrations. To detect these alterations, we will analyse the phenotypic profiling generated from image-based multiparametric single-cell analysis. The analysis and grouping of these parameters will be done through the use of data science tools like machine learning. This approach aims to develop a platform to identify subtle alterations by a variety of nanoparticles, contributing with the risk evaluation and the theoretical basis for the development of therapeutic management strategies. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES-LACERDA, THAIS; DE JESUS, MARCELO BISPO. Mechanisms of solid lipid nanoparticles-triggered signaling pathways in eukaryotic cells. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 220, p. 13-pg., 2022-10-19. (20/01218-3)

RIZOLI, CINTIA; DOS SANTOS, NATHALIA MEDINA; MAROSTICA JUNIOR, MARIO ROBERTO; DA CRUZ-HOFLING, MARIA ALICE; MENDONCA, MONIQUE CULTURATO PADILHA; DE JESUS, MARCELO BISPO. The therapeutic potential of reduced graphene oxide in attenuating cuprizone-induced demyelination in mice. Nanotechnology, v. 36, n. 2, p. 12-pg., 2025-01-13. (23/06143-0, 20/01218-3)

ALMEIDA, TUANNY LEITE; MOREIRA, ALINE FRANCISCA; DE OLIVEIRA, JHONES LUIZ; ROGERIO, CAROLINA BARBARA; KIIHL, SAMARA FLAMINI; FRACETO, LEONARDO FERNANDES; DE JESUS, MARCELO BISPO. A multiparametric and orthogonal approach indicates low toxicity for zein nanoparticles in a repellent formulation. TOXICOLOGY IN VITRO, v. 95, p. 9-pg., 2023-12-07. (20/01218-3, 15/06134-4, 14/03002-7)

MORAES-LACERDA, THAIS; GARCIA-FOSSA, FERNANDA; DE JESUS, MARCELO BISPO. Exploring the interplay between the TGF-β pathway and SLN-mediated transfection: implications for gene delivery efficiency in prostate cancer and non-cancer cells. Nanotechnology, v. 35, n. 32, p. 14-pg., 2024-08-05. (20/01218-3)

GARCIA-FOSSA, FERNANDA; CRUZ, MARIO COSTA; HAGHIGHI, MARZIEH; DE JESUS, MARCELO BISPO; SINGH, SHANTANU; CARPENTER, ANNE E.; CIMINI, BETH A.. Interpreting Image-based Profiles using Similarity Clustering and Single-Cell Visualization. CURRENT PROTOCOLS, v. 3, n. 3, p. 21-pg., 2023-03-01. (19/24033-1, 22/01483-4, 20/01218-3)

GARCIA-FOSSA, FERNANDA; MORAES-LACERDA, THAIS; RODRIGUES-DA-SILVA, MARIANA; DIAZ-ROHRER, BARBARA; SINGH, SHANTANU; CARPENTER, ANNE E.; CIMINI, BETH A.; DE JESUS, MARCELO BISPO. Live-cell painting: Image-based profiling in live cells using acridine orange. MOLECULAR BIOLOGY OF THE CELL, v. 36, n. 7, p. 11-pg., 2025-07-01. (19/24033-1, 23/06143-0, 22/01483-4, 20/01218-3)

OLIVIERI, PAULO H. JR JR; JESUS, MARCELO B.; NADER, HELENA B.; JUSTO, GISELLE Z.; SOUSA, ALIOSCKA A.. Cell-surface glycosaminoglycans regulate the cellular uptake of charged polystyrene nanoparticles. NANOSCALE, v. 14, n. 19, p. 14-pg., 2022-05-10. (20/01218-3, 21/00685-0, 15/03964-6)

DA COSTA, THYERRE SANTANA; DA SILVA, MARIANA RODRIGUES; BARBOSA, JULIO CESAR JERONIMO; DAS NEVES, UEDSON DA SILVA; DE JESUS, MARCELO BISPO; TASIC, LJUBICA. Biogenic silver nanoparticles' antibacterial activity and cytotoxicity on human hepatocarcinoma cells (Huh-7). RSC ADVANCES, v. 14, n. 4, p. 13-pg., 2024-01-10. (23/02338-0, 23/06874-4, 22/07854-4, 20/08615-8, 20/01218-3)