Multi-user equipment approved in grant 2017/17728-8, equipment High Resolution System for measuring the concentration of oxygen in solution, with fluorescence monitoring

• Grant number: 21/12964-0
• Support Opportunities: Multi-user Equipment Program
• Start date: February 01, 2022
• End date: January 31, 2029
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Roger Frigério Castilho
• Grantee: Roger Frigério Castilho
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/17728-8 - Mitochondrial function and dysfunction: implications for aging and associated diseases, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Analisadores; Processos Biológicos - Caracterização - Leitores de fluorescência (imageamento, placas); Caracterização de Materiais - Análises Químicas - Monitoramento de gases
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

In the last 40 years, it has become evident that mitochondria are involved in many cell functions besides energy conversion, such as the control of cell death via calcium and oxygen and nitrogen reactive species signaling. Elevated levels of calcium in the mitochondrial matrix stimulate the generation of reactive oxygen which promote the opening of an unspecific pore in the inner mitochondrial membrane, the permeability transition pore (PTP). Opening of PTP and mitochondrial redox state alterations are associated with cell senescence and death, as well as with the progression of degenerative diseases and with aging. In addition, there is compelling evidence that further studies on the control of PTP opening in aging may open avenues to a better understanding of factors determining longevity. Therefore, the major goal of this project is to advance the knowledge about the mechanisms by which mitochondria exert their regulatory roles in cell life and death and in aging. For this purpose, we plan to use several models (mice, rats, Caenorhabditis elegans, turtles and trypanosomes) to study mitochondrial functions involved in calcium homeostasis, redox (um)balance, longevity, aging and aging-related diseases. The specific objectives are grouped in four research lines: I) Nicotinamide nucleotides transhydrogenase, energy metabolism and cell viability; II) Mitochondria, dyslipidemias, atherosclerosis and obesity; III) Mitochondria and aging; and IV) Mitochondria and calcium homeostasis in trypanosomatides. Each model will be used to answer specific hypotheses in an integrated and multidisciplinar way with the participation of all principal investigators and collaborators. (AU)