Decoding the microbiota correlations of teeth indicated for endodontic retreatment and the complex interrelationship between mobiloma and resistome in oral site metagenomes

• Grant number: 21/13871-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2022
• End date: September 30, 2024
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Brenda Paula Figueiredo de Almeida Gomes
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Bruce J. Paster ; Tsute Chen

Abstract

The complex network that interconnects the sharing of genetic information between microbial communities is one of the main forces driving evolutionary processes. Through this powerful machinery operated by the mobiloma (i.e., set of mobile elements in a genome), microorganisms distribute genetic characteristics throughout ecosystems and living organisms. A significant example of this impressive web of life is the global spread of genes encoding antibiotic resistance. Despite posing a major threat to international public health, deciphering the principles that coordinate the evolution of multidrug-resistant bacteria remains a challenge. Currently, a range of works has revealed the potential of metagenomic approaches to understand and explore the dynamics and diversity of microbial communities and the correlation of resistance genes associated with the root canal microbiome, but to date there are no studies aimed at associating the root canal microbiota. cases indicated for endodontic retreatment and resistance strands, was performed. In this context, the objective of this project is to develop a systemic approach to analyze the antibiotic resistance network in oral cavity metagenomes associated with microorganisms found in root canal samples indicated for endodontic retreatment, integrating technologies to decode the distribution, abundance, association and statistical correlations of the resistome and mobiloma on a global scale from these data. Through laboratory approaches, bioinformatics and network science, the project's perspective is to unveil patterns of resistance genes prevalent in some groups of oral cavity data and the flow of their dissemination in microbial communities, as well as associations with the microbiota present in infections endodontic (IE). These data will be of fundamental importance for understanding the national panorama of dissemination of ARGs (Antibiotic Gene Resistance) in relation to the global challenge of controlling the development of superbugs and diseases arising from antibiotic resistance, paving the way for new technologies and health protocols with regarding the use of these drugs as well as the control of recurrent endodontic infection. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, BRENDA P. F. A.; BERBER, VANESSA B.; CHIARELLI-NETO, VITO M.; AVEIRO, EMELLY; CHAPOLA, RAFAELA C.; PASSINI, MAICON R. Z.; LOPES, ERICA M.; CHEN, TSUTE; PASTER, BRUCE J.. Microbiota present in combined endodontic-periodontal diseases and its risks for endocarditis. CLINICAL ORAL INVESTIGATIONS, v. 27, n. 8, p. 15-pg., 2023-07-04. (17/14912-2, 15/23479-5, 21/13871-6)

ARRUDA-VASCONCELOS, R.; CHIARELLI-NETO, V. M.; LOUZADA, L. M.; AVEIRO, E.; ALVES-SILVA, E. G.; DE-JESUS-SOARES, A.; FERRAZ, C. C. R.; ALMEIDA, J. F. A.; MARCIANO, M. A.; PECORARI, V. G. A.; et al. Quantitative analysis of culturable bacteria, levels of endotoxins, inflammatory mediators and substance P in teeth with symptomatic irreversible pulpitis and in teeth with vital normal pulp tissues. International Endodontic Journal, v. 56, n. 7, p. 10-pg., 2023-05-02. (21/13871-6, 15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)

JÉSSICA RODRIGUES RAMOS; JULIANA DELATORRE BRONZATO; ELOA CRISTINA BICEGO-PEREIRA; ADRIANA DE-JESUS-SOARES; MARINA ANGELICA MARCIANO; JOSÉ FLÁVIO AFFONSO ALMEIDA; CAIO CEZAR RANDI FERRAZ; BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES. Prevalence and reasons for referrals to the Endodontics Specialty Clinic at the Piracicaba Dental School. Brazilian Oral Research, v. 38, 2024-01-05. (21/13871-6, 15/23479-5, 17/25090-3)

GODOI-JR, EDERALDO P.; BRONZATO, JULIANA D.; FRANCISCO, PRISCILA A.; BICEGO-PEREIRA, ELOA C.; LOPES, ERICA M.; PASSINI, MAICON R. Z.; DE-JESUS-SOARES, ADRIANA; ALMEIDA, JOSE F. A.; MARCIANO, MARINA A.; FERRAZ, CAIO C. R.; et al. Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons. CLINICAL ORAL INVESTIGATIONS, v. 27, n. 5, p. 16-pg., 2023-03-31. (19/14448-0, 17/16516-7, 17/25090-3, 21/13871-6, 21/14570-0)

CHIARELLI-NETO, VITO M.; DE AVEIRO, EMELLY; BRONZATO, JULIANA D.; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; GODOI, EDERALDO P.; LOPES, ERICA M.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; et al. Clinical evaluation of culturable bacteria, endotoxins and lipoteichoic acid in teeth with vital normal pulp tissues. AUSTRALIAN ENDODONTIC JOURNAL, v. N/A, p. 9-pg., 2023-08-01. (21/13871-6, 15/23479-5, 19/14448-0, 21/14570-0, 17/25242-8, 17/25090-3, 19/19300-0)