Ultrastructural characterization of bioactive nanoscaffolds

• Grant number: 22/03056-6
• Support Opportunities: Research Grants - Initial Project
• Start date: March 01, 2023
• End date: February 29, 2028
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Emerson Rodrigo da Silva
• Grantee: Emerson Rodrigo da Silva
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: clovis ryuichi nakaie ; Renata Naporano Bicev
• Associated research grant(s): 24/07196-2 - Multiuser equipment approved in the grant 22/03056-6: atomic force microscope, AP.EMU
• Associated scholarship(s): 25/11824-1 - Nanoscale Organization of Peptide Hydrogel Matrices: Topography, Cell Adhesion, and Nanomechanical Properties, BP.DR; 25/11693-4 - High-resolution topographic mapping of cells adhered to hydrogel matrices, BP.IC

Abstract

Nanostructured matrices obtained from the self-organization of biomolecules are a promising class to formulate the next generation of biomaterials. In this context, three-dimensional scaffolds consisting of nanoscale fibrillar networks that mimic the biological environment are among the most attractive systems for biotechnological applications. Obviously, detailed knowledge of the spatial organization of these nanoscale arrangements is a crucial aspect for their full development and application. This project aims to develop and consolidate a research line related to the structural characterization of nanoscale scaffolds endowed with bioactivity. The matrices developed here will be peptide-based hydrogels capable of reproducing the microenvironment found in biological tissues and modulating cellular responses. Particularly, we will formulate self-assembled matrices containing cell adhesion motifs and cell penetrating peptides. The ultrastructure of these architectures will be analyzed by a wide range of biophysical techniques, with special emphasis on atomic force microscopy (AFM). This is an integrated project that, by training undergraduate and graduate students, will lead to the implementation of the AFM technique applied to the analysis of biological materials at the Sao Paulo campus of the Federal University of Sao Paulo. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FACTORI, IRINA M.; DA SILVA, EMERSON R.; GERBELLI, BARBARA B.; LIBERATO, MICHELLE S.; DE ARAUJO, DANIELE RIBEIRO; ALVES, WENDEL A.. PVA/titanate nanotube nanocomposites: Evaluating the required structural properties for their performance as macromolecule delivery systems. Journal of Applied Polymer Science, v. 141, n. 46, p. 13-pg., 2024-09-05. (22/03056-6, 14/50867-3, 22/14753-0)

CALIARI, ANA B.; BICEV, RENATA N.; DA SILVA, CAROLINE C.; DE SOUZA, SINVAL E. G.; DA SILVA, MARTA G.; SOUZA, LOUISE E. A.; DE MELLO, LUCAS R.; HAMLEY, IAN W.; MOTTA, GUACYARA; DEGROUARD, JERIL; et al. Self-assembly, cytocompatibility, and interactions of desmopressin with sodium polystyrene sulfonate. SOFT MATTER, v. N/A, p. 17-pg., 2024-11-19. (21/04885-3, 22/03056-6, 23/18385-8, 17/22047-0, 19/19719-1)

DE MELLO, LUCAS RODRIGUES; HONDA, TAMISA SEEKO BANDEIRA; HAN, SANG WON; CASTELLETTO, VALERIA; HAMLEY, IAN WILLIAM; POROSK, LY; LANGEL, ULO; DA SILVA, EMERSON RODRIGO. Structure-activity relationships of DNA nanocarriers based on the amphipathic cell penetrating peptide transportan 10. RSC PHARMACEUTICS, v. 1, n. 5, p. 18-pg., 2024-12-10. (19/19719-1, 22/03056-6)

POROSK, LY; HARK, HELERI HEIKE; BICEV, RENATA NAPORANO; GAIDUTSIK, ILJA; NEBOGATOVA, JEKATERINA; ARMOLIK, EGER-JASPER; ARUKUUSK, PIRET; DA SILVA, EMERSON RODRIGO; LANGEL, ULO. Aggregation Limiting Cell-Penetrating Peptides Derived from Protein Signal Sequences. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 5, p. 20-pg., 2023-03-01. (22/03056-6)

LOURENCO, THIAGO C.; DE MELLO, LUCAS R.; ICIMOTO, MARCELO Y.; BICEV, RENATA N.; HAMLEY, IAN W.; CASTELLETTO, VALERIA; NAKAIE, CLOVIS R.; DA SILVA, EMERSON R.. DNA-templated self-assembly of bradykinin into bioactive nanofibrils. SOFT MATTER, v. 19, n. 26, p. 11-pg., 2023-06-12. (21/04885-3, 22/03056-6, 19/19719-1, 21/10092-6)

FERREIRA, MARIANA M. L.; DE SOUZA, SINVAL E. G.; DA SILVA, CAROLINE C.; SOUZA, LOUISE E. A.; BICEV, RENATA N.; DA SILVA, EMERSON R.; NAKAIE, CLOVIS R.. Pyroglutamination-Induced Changes in the Physicochemical Features of a CXCR4 Chemokine Peptide: Kinetic and Structural Analysis. BIOCHEMISTRY, v. 62, n. 17, p. 11-pg., 2023-08-04. (22/03056-6, 21/04885-3)