Bioactive peptide matrices: from molecular structure to biomedical applications

• Grant number: 19/19719-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2020
• End date: May 01, 2024
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Emerson Rodrigo da Silva
• Grantee: Lucas Rodrigues de Mello
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated scholarship(s): 21/10092-6 - Development of bioactive peptide matrices: from molecular structure to biomedical applications, BE.EP.DR

Abstract

This project aims to produce and characterize novel nanostructured scaffolds obtained from self-assembling bioactive peptides. Our focus will be on sequences belonging to three major classes of bioactive compounds: cell penetrating peptides, constituents of extracellular matrix (collagen- and elastin-like sequences) and anti-inflammatory peptides. We will seek for answers to fundamental questions related to the self-assembly pathway of these matrices and to their supramolecular properties. The structure of these scaffolds in the presence of model drugs will be investigated. A set of advanced techniques encompassing infrared nanospectroscopy combined with Atomic Force Microscopy (AFM-IR), Small-Angle X-ray Scattering (SAXS) and Coherent Anti-Stoke Raman Scattering microscopy (CARS) will be used in the endeavor of providing detailed information on the organization of these systems. In addition to deep structural characterization, we aim to test bioactivity to determine if functions exhibited by molecular blocks are preserved or even enhanced upon organization into superstructures. Based on both structural and bioactivity data, we aim to establish structure-activity relationships and contribute for optimizing the formulation of biomedical materials based on these bioactive molecules. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE MELLO, LUCAS R.; CARRASCOSA, VINICIUS; REBELATO, EDUARDO; JULIANO, MARIA A.; HAMLEY, IAN W.; CASTELLETTO, VALERIA; VASSILIADES, SANDRA V.; ALVES, WENDEL A.; NAKAIE, CLOVIS R.; DA SILVA, EMERSON R.. Nanostructure Formation and Cell Spheroid Morphogenesis of a Peptide Supramolecular Hydrogel. Langmuir, v. 38, n. 11, p. 12-pg., 2022-03-22. (18/23269-9, 19/19719-1, 17/02317-2, 21/10092-6, 14/50867-3)

ROSA, ELISABETTA; DE MELLO, LUCAS; CASTELLETTO, VALERIA; DALLAS, MARK L.; ACCARDO, ANTONELLA; SEITSONEN, JANI; HAMLEY, IAN W.. Cell Adhesion Motif-Functionalized Lipopeptides: Nanostructure and Selective Myoblast Cytocompatibility. Biomacromolecules, v. N/A, p. 12-pg., 2022-12-15. (19/20907-7, 21/10092-6, 19/19719-1)

CINEL, VICTOR DAL POSOLO; TAKETA, THIAGO BEZERRA; DE CARVALHO, BRUNA GREGATTI; DE LA TORRE, LUCIMARA GAZIOLA; DE MELLO, LUCAS RODRIGUES; DA SILVA, EMERSON RODRIGO; HAN, SANG WON. Microfluidic encapsulation of nanoparticles in alginate microgels gelled via competitive ligand exchange crosslinking. Biopolymers, v. 112, n. 7, JUL 2021. (18/19537-8, 18/18523-3, 19/19719-1, 18/06635-1, 15/20206-8)

DE MELLO, LUCAS R.; POROSK, LY; LOURENCO, THIAGO C.; GARCIA, BIANCA B. M.; COSTA, CARLOS A. R.; HAN, SANG W.; DE SOUZA, JULIANA S.; LANGEL, ULO; DA SILVA, EMERSON R.. Amyloid-like Self-Assembly of a Hydrophobic Cell-Penetrating Peptide and Its Use as a Carrier for Nucleic Acids. ACS APPLIED BIO MATERIALS, v. 4, n. 8, p. 6404-6416, AUG 16 2021. (19/20907-7, 19/19719-1)

ROSA, ELISABETTA; DIAFERIA, CARLO; DE MELLO, LUCAS; SEITSONEN, JANI; HAMLEY, IAN W.; ACCARDO, ANTONELLA. Self-assembled aggregates based on cationic amphiphilic peptides: structural insight. SOFT MATTER, v. 19, n. 25, p. 11-pg., 2023-05-23. (19/19719-1, 19/20907-7, 21/10092-6)

LOURENCO, THIAGO C.; DE MELLO, LUCAS R.; ICIMOTO, MARCELO Y.; BICEV, RENATA N.; HAMLEY, IAN W.; CASTELLETTO, VALERIA; NAKAIE, CLOVIS R.; DA SILVA, EMERSON R.. DNA-templated self-assembly of bradykinin into bioactive nanofibrils. SOFT MATTER, v. 19, n. 26, p. 11-pg., 2023-06-12. (21/04885-3, 22/03056-6, 19/19719-1, 21/10092-6)