Formulation of tailor-made enzymatic consortiums for the degradation of the cell wall of sugarcane varieties

• Grant number: 23/01547-5
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2023
• End date: July 31, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Agreement: CNPq
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Maria de Lourdes Teixeira de Moraes Polizeli
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol, AP.TEM
• Associated scholarship(s): 23/09627-8 - Formulation of tailor-made enzymatic consortiums for the degradation of the cell wall of sugarcane varieties, BP.JD

Abstract

The INCT-Bioethanol has made significant progress in studies of the composition of the sugarcane cell wall in recent years, providing important information on the structure of polymers and how enzymes access the wall during hydrolysis, aiming to increase cellulose conversion and hemicellulose into monosaccharides. The present proposal aims to integrate this knowledge to develop enzymatic consortiums containing free and immobilized enzymes to hydrolyze the biomass obtained from hybrid varieties of sugarcane Energia and SP80-3280. To enable the execution of this work plan, considering its scope and goals, this proposal is based on three main stages: I) formulating tailor-made enzymatic consortia from recombinant holocellulases for application in the hydrolysis of sugarcane varieties of sugar; II) preparing new nanoporous magnetic supports from biodegradable matrices, such as starch and cellulose; III) co-immobilize holocellulases on different supports and apply the different immobilized formulations in the hydrolysis of sugarcane varieties. It is expected to obtain different formulations of enzymes with high efficiency in the hydrolysis of the biomass of sugarcane varieties, as well as for other grasses under study within the INCT-Bioethanol. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAGHETTO DE ALMEIDA, PAULA; ALNOCH, ROBSON CARLOS; PINHEIRO, VANESSA ELISA; PEREIRA GIMENEZ, MARITA; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Biochemical Characterization of a Novel Thermostable 1,4-α-Glucoamylase from Aspergillus brasiliensis Strain Isolated in the Brazilian Atlantic Forest. Applied Biochemistry and Biotechnology, v. 196, n. 10, p. 20-pg., 2024-03-21. (13/01437-3, 14/50884-5, 20/00081-4, 18/07522-6, 23/09627-8, 23/01547-5)

ORTOLAN, GUILHERME GUIMARAES; CONTATO, ALEX GRACA; ARANHA, GUILHERME MAURO; SALGADO, JOSE CARLOS SANTOS; ALNOCH, ROBSON CARLOS; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization. REACTIONS, v. 5, n. 3, p. 16-pg., 2024-09-01. (17/25862-6, 14/50884-5, 18/07522-6, 19/21989-7, 22/06275-0, 23/09627-8, 23/01547-5)

ALVES, GABRIELA S.; DE ANDRADES, DIANDRA; SALGADO, JOSE C. S.; MARIANO, CLARA B.; BERTO, GABRIELA LEILA; SEGATO, FERNANDO; AYUB, MARCO ANTONIO ZACHIA; WARD, RICHARD J.; ALNOCH, ROBSON C.; POLIZELI, MARIA DE LOURDES T. M.. Homologous expression, purification, and characterization of a recombinant acetylxylan esterase from Aspergillus nidulans. International Journal of Biological Macromolecules, v. 280, p. 12-pg., 2024-10-11. (20/15510-8, 14/50884-5, 18/07522-6, 19/21989-7, 23/09627-8, 23/01547-5)