Metagenomic and metasecretomic analyses of an enrichment microbial consortium of anaerobic fungi to the degradation of sugarcane bagasse: prospection of cellulosomas and lignocellolytic enzymes

• Grant number: 15/23279-6
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): June 01, 2016
• Effective date (End): December 10, 2020
• Field of knowledge: Biological Sciences - Genetics
• Principal Investigator: Fábio Márcio Squina
• Grantee: Geizecler Tomazetto
• Home Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
• Associated research grant: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models, AP.BIOEN.TEM
• Associated scholarship(s): 18/23826-5 - Cellulosomes - a versatile enzymatic scaffold platform for bioconversion of lignocellulosic biomass into value-added fine chemicals, BE.EP.PD

Abstract

Second generation of biofuel derived from lignocellulosic biomass is a trend for future of the fuel market. In Brazil, there are several of studies to use sugarcane bagasse as carbon resource for bioethanol production. Rumen anaerobic fungi has its profile of lignocellulolytic enzymes yet unexplored, beyond there is lack of sufficient understanding of synergism and microbial interaction that occur during the process of biomass degradation within rumen. Likewise, rumen microbiome also represents a resource for prospection of fungal cellulosomes due to several dockerin sequences present in glycoside hydrolases. In contrast to bacterial cellulosomes, only few studies described the architecture and enzymes anchoring mechanism in fungal cellulosomes. In this context, the goal of this project will be to employ bovine rumen sample to construct a fungal anaerobic consortium degrading of sugarcane bagasse. The anaerobic fungal population within microbial consortium will be quantified by Real-Time PCR. Subsequently, the genetic and extracellular enzymes contents will be analyzed by metagenomic and metasecretomic approaches. Obtained datasets compilation will provide information for identification and reconstruction of fungal cellulosomes (in silico). The fungal cellulosomes identified will be cloned and expressed in Aspergillus nidulans. Finally, the enzymes revealed will be evaluated for its potential of complementing commercial lignocellulolytic cocktail.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAES, EDUARDO C.; ALVAREZ, THABATA M.; PERSINOTI, GABRIELA F.; TOMAZETTO, GEIZECLER; BRENELLI, LIVIA B.; PAIXAO, DOUGLAS A. A.; EMATSU, GABRIELA C.; ARICETTI, JULIANA A.; CALDANA, CAMILA; DIXON, NEIL; BUGG, TIMOTHY D. H.; SQUINA, FABIO M. Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization. BIOTECHNOLOGY FOR BIOFUELS, v. 11, MAR 22 2018. Web of Science Citations: 10.