Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties

Author(s):Show less -

Alvaredo Paixao, Douglas Antonio ^[1] ; Tomazetto, Geizecler ^[2] ; Sodre, Victoria Ramos ^{[3, 4]} ; Goncalves, Thiago A. ^{[3, 4]} ; Uchima, Cristiane Akemi ^[1] ; Buchli, Fernanda ^[1] ; Alvarez, Thabata Maria ^[5] ; Persinoti, Gabriela Felix ^[1] ; da Silva, Marcio Jose ^[6] ; Bragatto, Juliano ^[1] ; Liberato, Marcelo Vizona ^{[4, 1]} ; Franco Cairo, Joao Paulo L. ^{[3, 4]} ; Paes Leme, Adriana Franco ^[7] ; Squina, Fabio Marcio ^[4]

Affiliation

^[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biorenovaveis, Campinas, SP - Brazil

^[2] Aarhus Univ, Dept Biol & Chem Engn BCE, DK-8200 Aarhus - Denmark

^[3] Univ Estadual Campinas, Inst Biol, Dept Bioquim & Biol Tecidual, Campinas, SP - Brazil

^[4] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil

^[5] Univ Posit, Grad Programme Ind Biotechnol, Curitiba, Parana - Brazil

^[6] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, Campinas, SP - Brazil

^[7] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Biociencias, Campinas, SP - Brazil

• Document type: Journal article
• Source: Enzyme and Microbial Technology; v. 148, AUG 2021.
• Web of Science Citations: 0

Abstract

Although lignocellulose is the most abundant and renewable natural resource for biofuel production, its use remains under exploration because of its highly recalcitrant structure. Its deconstruction into sugar monomers is mainly driven by carbohydrate-active enzymes (CAZymes). To develop highly efficient and fast strategies to discover biomass-degrading enzymes for biorefinery applications, an enrichment process combined with integrative omics approaches was used to identify new CAZymes. The lignocellulolytic-enriched mangrove microbial community (LignoManG) established on sugarcane bagasse (SB) was enriched with lignocellulolytic bacteria and fungi such as Proteobacteria, Bacteroidetes, Basidiomycota, and Ascomycota. These microbial communities were able to degrade up to 55 % of the total SB, indicating the production of lignocellulolytic enzymes. Metagenomic analysis revealed that the LignoManG harbors 18.042 CAZyme sequences such as of cellulases, hemicellulases, carbohydrate esterases, and lytic polysaccharide monooxygenase. Similarly, our metaproteomic analysis depicted several enzymes from distinct families of different CAZy families. Based on the LignoManG data, a xylanase (coldXynZ) was selected, amplified, cloned, expressed, and biochemically characterized. The enzyme displayed psicrofilic properties, with the highest activity at 15 degrees C, retaining 77 % of its activity when incubated at 0 degrees C. Moreover, molecular modeling in silico indicated that coldXynZ is composed of a TIM barrel, which is a typical folding found in the GH10 family, and displayed similar structural features related to cold-adapted enzymes. Collectively, the data generated in this study represent a valuable resource for lignocellulolytic enzymes with potential biotechnological applications. (AU)

• FAPESP's process: 12/19040-0 - Characterization of gene expression of Neurospora crassa in response to deconstruction of sugar cane bagasse
• Grantee: Cristiane Akemi Uchima
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 14/04105-4 - Structural and functional characterization of new cellulases, focusing in the relation between catalytic domains and CBMs
• Grantee: Marcelo Vizoná Liberato
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 16/09950-0 - Functional, structural characterizations and biotechnological application of lytic polysaccharide monooxygenases from the lower termite Coptotermes gestroi
• Grantee: João Paulo Lourenço Franco Cairo
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 15/23279-6 - Metagenomic and metasecretomic analyses of an enrichment microbial consortium of anaerobic fungi to the degradation of sugarcane bagasse: prospection of cellulosomas and lignocellolytic enzymes
• Grantee: Geizecler Tomazetto
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/18101-1 - Development of the CRISPR/Cas9 system in Rhodococcus jostii RHA1 for production of high-value chemicals from lignin streams
• Grantee: Victoria Ramos Sodré de Castro
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/16089-1 - Production of lignin chains, metagenomic screening and biocatalytic conversion of lignin to ferulic acid
• Grantee: Thiago Augusto Gonçalves
• Support type: Scholarships in Brazil - Post-Doctorate