Characterization of gene expression of Neurospora crassa in response to deconstruction of sugar cane bagasse

• Grant number: 12/19040-0
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): January 01, 2013
• Effective date (End): August 31, 2014
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal researcher: Fábio Márcio Squina
• Grantee: Cristiane Akemi Uchima
• Home Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
• Associated research grant: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP
• Associated scholarship(s): 14/10351-8 - Phenotype characterization of Neurospora crassa deletion strains grown on avicel and sugarcane bagasse, BE.EP.PD

Abstract

The continuous use of fossil fuels reflects in global warming and generates worries regarding its availability. Bioethanol appears as an attractive option of renewable and cleaner energy source. The increase in Brazilian bioethanol production may come from sugar cane bagasse, which is sustainable and suitable to be converted into biofuels. The cristallinity of the plant cell wall can be circumvented with physical or chemical pre treatments, together with the use of enzyme mixtures. The development of these mixtures is the main bottleneck in conversion of lignocellulosic biomass into ethanol due to its high cost. In nature, some microorganism efficiently degrades plant biomass, as the filamentous fungus Neurospora crassa. The objective of this pos-doctoral research is to characterize the gene expression of N. crassa, in response to its growth in medium containing sugar cane bagasse. The secretome of this fungus will be analyzed by proteomic, qRT-PCR and biochemical techniques, in the attempt to understand the mechanisms utilized by N. crassa in the degradation of sugar cane bagasse. Furthermore, this research may help the development of more efficient enzyme mixtures that can lead Brazil in an outstanding position not only in relation to the production of second generation biofuels, but also in basic scientific research on this strategic subject.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVAREDO PAIXAO, DOUGLAS ANTONIO; TOMAZETTO, GEIZECLER; SODRE, VICTORIA RAMOS; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE AKEMI; BUCHLI, FERNANDA; ALVAREZ, THABATA MARIA; PERSINOTI, GABRIELA FELIX; DA SILVA, MARCIO JOSE; BRAGATTO, JULIANO; LIBERATO, MARCELO VIZONA; FRANCO CAIRO, JOAO PAULO L.; PAES LEME, ADRIANA FRANCO; SQUINA, FABIO MARCIO. Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties. Enzyme and Microbial Technology, v. 148, AUG 2021. Web of Science Citations: 0.

DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; BATTAGLIA, EVY; BAYRAM, OZGUR; BENOCCI, TIZIANO; BRAUS-STROMEYER, SUSANNA A.; CALDANA, CAMILA; CANOVAS, DAVID; CERQUEIRA, GUSTAVO C.; CHEN, FUSHENG; CHEN, WANPING; CHOI, CINDY; CLUM, ALICIA; CORREA DOS SANTOS, RENATO AUGUSTO; DE LIMA DAMASIO, ANDRE RICARDO; DIALLINAS, GEORGE; EMRI, TAMAS; FEKETE, ERZSEBET; FLIPPHI, MICHEL; FREYBERG, SUSANNE; GALLO, ANTONIA; GOURNAS, CHRISTOS; HABGOOD, ROB; HAINAUT, MATTHIEU; LAURA HARISPE, MARIA; HENRISSAT, BERNARD; HILDEN, KRISTIINA S.; HOPE, RYAN; HOSSAIN, ABEER; KARABIKA, EUGENIA; KARAFFA, LEVENTE; KARANYI, ZSOLT; KRASEVEC, NADA; KUO, ALAN; KUSCH, HARALD; LABUTTI, KURT; LAGENDIJK, ELLEN L.; LAPIDUS, ALLA; LEVASSEUR, ANTHONY; LINDQUIST, ERIKA; LIPZEN, ANNA; LOGRIECO, ANTONIO F.; MACCABE, ANDREW; MAKELA, MIIA R.; MALAVAZI, IRAN; MELIN, PETTER; MEYER, VERA; MIELNICHUK, NATALIA; MISKEI, MARTON; MOLNAR, AKOS P.; MULE, GIUSEPPINA; NGAN, CHEW YEE; OREJAS, MARGARITA; OROSZ, ERZSEBET; OUEDRAOGO, JEAN PAUL; OVERKAMP, KARIN M.; PARK, HEE-SOO; PERRONE, GIANCARLO; PIUMI, FRANCOIS; PUNT, PETER J.; RAM, ARTHUR F. J.; RAMON, ANA; RAUSCHER, STEFAN; RECORD, ERIC; RIANO-PACHON, DIEGO MAURICIO; ROBERT, VINCENT; ROEHRIG, JULIAN; RULLER, ROBERTO; SALAMOV, ASAF; SALIH, NADHIRA S.; SAMSON, ROB A.; SANDOR, ERZSEBET; SANGUINETTI, MANUEL; SCHUTZE, TABEA; SEPCIC, KRISTINA; SHELEST, EKATERINA; SHERLOCK, GAVIN; SOPHIANOPOULOU, VICKY; SQUINA, FABIOM.; SUN, HUI; SUSCA, ANTONIA; TODD, RICHARD B.; TSANG, ADRIAN; UNKLES, SHIELA E.; VAN DE WIELE, NATHALIE; VAN ROSSEN-UFFINK, DIANA; DE CASTRO OLIVEIRA, JULIANA VELASCO; VESTH, TAMMI C.; VISSER, JAAP; YU, JAE-HYUK; ZHOU, MIAOMIAO; ANDERSEN, MIKAEL R.; ARCHER, DAVID B.; BAKER, SCOTT E.; BENOIT, ISABELLE; BRAKHAGE, AXEL A.; BRAUS, GERHARD H.; FISCHER, REINHARD; FRISVAD, JENS C.; GOLDMAN, GUSTAVO H.; HOUBRAKEN, JOS; OAKLEY, BERL; POCSI, ISTVAN; SCAZZOCCHIO, CLAUDIO; SEIBOTH, BERNHARD; VANKUYK, PATRICIA A.; WORTMAN, JENNIFER; DYER, PAUL S.; GRIGORIEV, IGOR V. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biology, v. 18, FEB 14 2017. Web of Science Citations: 108.

FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; VIDAL, RAMON O.; COSTA, FERNANDO F.; COSTA-LEONARDO, ANA M.; PAES LEME, ADRIANA F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, OCT 13 2016. Web of Science Citations: 8.

HAHN SCHNEIDER, WILLIAN DANIEL; GONCALVES, THIAGO AUGUSTO; UCHIMA, CRISTIANE AKEMI; COUGER, MATTHEW BRIAN; PRADE, ROLF; SQUINA, FABIO MARCIO; PINHEIRO DILLON, ALDO JOSE; CAMASSOLA, MARLI. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. BIOTECHNOLOGY FOR BIOFUELS, v. 9, MAR 17 2016. Web of Science Citations: 23.

FRANCO CAIRO, JOAO PAULO L.; OLIVEIRA, LEANDRO C.; UCHIMA, CRISTIANE A.; ALVAREZ, THABATA M.; CITADINI, ANA PAULA DA S.; COTA, JUNIO; LEONARDO, FLAVIA COSTA; COSTA-LEONARDO, ANA M.; CARAZZOLLE, MARCELO F.; COSTA, FERNANDO F.; PEREIRA, GONCALO A. G.; SQUINA, FABIO M. Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi. Insect Biochemistry and Molecular Biology, v. 43, n. 10, p. 970-981, OCT 2013. Web of Science Citations: 16.