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Characterization of gene expression of Neurospora crassa in response to deconstruction of sugar cane bagasse

Grant number: 12/19040-0
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Start date: January 01, 2013
End date: August 31, 2014
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Fábio Márcio Squina
Grantee:Cristiane Akemi Uchima
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
Associated research grant:08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP
Associated scholarship(s):14/10351-8 - Phenotype characterization of Neurospora crassa deletion strains grown on avicel and sugarcane bagasse, BE.EP.PD

Abstract

The continuous use of fossil fuels reflects in global warming and generates worries regarding its availability. Bioethanol appears as an attractive option of renewable and cleaner energy source. The increase in Brazilian bioethanol production may come from sugar cane bagasse, which is sustainable and suitable to be converted into biofuels. The cristallinity of the plant cell wall can be circumvented with physical or chemical pre treatments, together with the use of enzyme mixtures. The development of these mixtures is the main bottleneck in conversion of lignocellulosic biomass into ethanol due to its high cost. In nature, some microorganism efficiently degrades plant biomass, as the filamentous fungus Neurospora crassa. The objective of this pos-doctoral research is to characterize the gene expression of N. crassa, in response to its growth in medium containing sugar cane bagasse. The secretome of this fungus will be analyzed by proteomic, qRT-PCR and biochemical techniques, in the attempt to understand the mechanisms utilized by N. crassa in the degradation of sugar cane bagasse. Furthermore, this research may help the development of more efficient enzyme mixtures that can lead Brazil in an outstanding position not only in relation to the production of second generation biofuels, but also in basic scientific research on this strategic subject.

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biology, v. 18, . (14/06923-6, 12/20549-4, 12/19040-0, 11/08945-9, 14/10351-8, 14/11766-7)
FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; et al. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, . (15/06971-3, 14/20576-7, 14/50371-8, 12/19040-0, 14/10351-8, 11/20977-3, 13/03061-0, 10/11469-1, 08/58037-9)
FRANCO CAIRO, JOAO PAULO L.; OLIVEIRA, LEANDRO C.; UCHIMA, CRISTIANE A.; ALVAREZ, THABATA M.; CITADINI, ANA PAULA DA S.; COTA, JUNIO; LEONARDO, FLAVIA COSTA; COSTA-LEONARDO, ANA M.; CARAZZOLLE, MARCELO F.; COSTA, FERNANDO F.; et al. Deciphering the synergism of endogenous glycoside hydrolase families 1 and 9 from Coptotermes gestroi. Insect Biochemistry and Molecular Biology, v. 43, n. 10, p. 970-981, . (12/19040-0, 11/08609-9, 11/20977-3, 11/13242-7, 10/11469-1, 08/58037-9)
DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. GENOME BIOLOGY, v. 18, p. 45-pg., . (11/08945-9, 14/06923-6, 12/19040-0, 14/10351-8, 14/11766-7, 12/20549-4)
ALVAREDO PAIXAO, DOUGLAS ANTONIO; TOMAZETTO, GEIZECLER; SODRE, VICTORIA RAMOS; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE AKEMI; BUCHLI, FERNANDA; ALVAREZ, THABATA MARIA; PERSINOTI, GABRIELA FELIX; DA SILVA, MARCIO JOSE; BRAGATTO, JULIANO; et al. Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties. Enzyme and Microbial Technology, v. 148, . (12/19040-0, 15/50590-4, 14/04105-4, 16/09950-0, 15/23279-6, 18/18101-1, 17/16089-1)
HAHN SCHNEIDER, WILLIAN DANIEL; GONCALVES, THIAGO AUGUSTO; UCHIMA, CRISTIANE AKEMI; COUGER, MATTHEW BRIAN; PRADE, ROLF; SQUINA, FABIO MARCIO; PINHEIRO DILLON, ALDO JOSE; CAMASSOLA, MARLI. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (14/06923-6, 08/58037-9, 12/19040-0)