Molecular and Biochemical Analysis of Responses to Stress in Filamentous Fungi

• Grant number: 96/01428-7
• Support type: Research Projects - Thematic Grants
• Duration: November 01, 1996 - April 30, 2001
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Hector Francisco Terenzi
• Grantee: Hector Francisco Terenzi
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated grant(s): 00/00613-2 - The rcaA gene from Aspergillus nidulans is required for camptothecin resistance and related to the cellular DNA damage reponse, AR.EXT; 99/05885-1 - Cees Amjj Van Den Hondel | TNO Nutrition and Food Research Institute - Holanda, AV.EXT; 98/15849-0 - David Perlin | New York Public Health Research Institute - Estados Unidos, AV.EXT; 98/16579-6 - Catalase activity is necessary to heat-shock recovery in Aspergillus nidulans germilings, AR.EXT

Abstract

Coning and characterization of ubiquitin expression in A. nidulans under different stress conditions. Abnormal proteins arise continuously during any cellular stress process to avoid toxic levels abnormal proteins must be removed. A variety of biochemical and genetic evidence indicates that ubiquitin conjugation is a obligatory step in protein predegradation. We are interested in studying ubiquitin expression during different cellular stress processes in Aspergillus nidulans the main goals are: (i) cloning of the ubiquitin encoding genes of A. nidulans; (ii) characterization of ubiquitin gene expression under different stress conditions, and (iii) deletion or gene disruption of the ubiquitin gene. (AU)