Study of the virus-vector interaction and the mechanisms involved in modulating the dynamics of viral transmission

• Grant number: 22/03645-1
• Support Opportunities: Research Projects - Thematic Grants
• Start date: June 01, 2023
• End date: May 31, 2028
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Maurício Lacerda Nogueira
• Grantee: Maurício Lacerda Nogueira
• Host Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil
• Associated researchers: Adriano Pinter dos Santos ; Camila Lorenz ; Cássia Fernanda Estofolete ; Francisco Chiaravalloti Neto ; João Pessoa Araújo Junior ; João Trindade Marques ; Juliana Telles de Deus ; Margareth Regina Dibo ; Mauro Martins Teixeira ; Nikos Vasilakis ; Rafael Elias Marques Pereira Silva ; Regiane Maria Tironi de Menezes ; Scott C Weaver
• Associated research grant(s): 25/00398-1 - Multi-user equipament aprroved in grant 2022/03645-1, instrumento King Fisher extração de DNA ENA 96 DW HEAD, AP.EMU; 24/02259-6 - Vector competence of Aedes mosquitoes for arboviruses mediated by microorganisms: a study of vector biology and its influence on the occurrence of epidemics., AP.R
• Associated scholarship(s): 24/20412-6 - Viral Metagenomics: Exploring RNA viruses in Human and Vectors in the Amazon Basin., BP.DR; 24/22180-5 - INVESTIGATION OF THE INTERFERENCE OF GUAPIAÇU VIRUS, AN INSECT-SPECIFIC ORTHOFLAVIVIRUS, IN THE MULTIPLICATION AND TRANSMISSION CYCLE IN MEDICALLY IMPORTANT ARBOVIRUSES, BP.DR; 25/03512-0 - Collection and management of culicidae in urban, periurban and silvatic areas of the São José do Rio Preto, São Paulo, Brazilian, BP.TT; + associated scholarships 24/23108-6 - Identification and maintenance of vector colonies recently collected in urban, peri-urban, and wild areas of the municipality of São José do Rio Preto - SP., BP.TT; 24/19143-0 - Analysis of arbovirus circulation in mosquitoes of the genus Aedes in São José do Rio Preto, São Paulo, Brazil, BP.TT; 24/01633-1 - Collection and management of culicidae in urban, periurban and silvatic areas of the São José do Rio Preto, São Paulo, Brazilian, BP.TT; 24/00546-8 - Identification and maintenance of colonies of recently collected vectors in urban, periurban and wild areas of the municipality of São José do Rio Preto - SP, BP.TT; 23/14670-0 - Impact of the immune genes expression and the regulation mechanisms modulated by small-interfering RNAs in the antiviral response of Aedes mosquitoes., BP.PD - associated scholarships

Abstract

Arboviruses constitute one of the most diverse groups of RNA viruses, transmitted mainly by hematophagous insects, especially mosquitoes belonging to the Aedes genus. In South America, Brazil is considered an epicenter for emerging and re-emerging arboviruses diseases since outbreaks or epidemics of DENV1-4, YFV, MAYV, ROCV, SLEV, OROV, CHIKV, and ZIKV have been reported. The spread of these diseases is widely associated with factors that contribute to vector dispersion, as well as intrinsic characteristics of the species, that can influence vector competence and, consequently, arbovirus world-spread. Thus, in this project, we intend to investigate the complex, interconnected relationship between virus-vector, and provide insights on how to better proceed in the face of recurrent epidemics, which significantly affect the public health of several Brazilian municipalities, how São José do Rio Preto city (SJRP). São José do Rio Preto is a city from Northwest São Paulo State, with frequently occurring DENV epidemics. The DENV was introduced in SJRP in 1990 and from then on successive epidemics occurred in 2010, 2013, 2015, 2016 and 2019. Thus, the SJRP presents the other arboviruses' circulation, such as ZIKV and CHIKV, although epidemics never observed. In front of this, the hypothesis of this study is how the relationship between different factors of vector biology can affect the dynamic of arbovirus transmission in SJRP and what the influence in the recurrent local DENV epidemics and the absence of ZIKV and CHIKV outbreaks. Therefore, in this project, we propose investigating the different factors related to vector competence that affect the genetic variability in determinate mosquito's population; the fitness viral that is arbovirus linage current dependent; vector microbiome and virus persistence in the vector, that can be modulate by gene expression and viral replication and contributing of viral circulation in unfavorable period. For this, we will realize successive collections of mosquitos, eggs then adults, in three different areas: urban, periurban and sylvatic. Thus, with the collection of mosquitoes, we will build Aedes mosquitoes colonies native from SJRP and these are established in appropriated insectary for posterior vector competence assays, how influence of different microorganisms and ISVs, fitness viral and gene expression and regulation viral replication assays. The adult mosquitos will be molecular tested for arboviroses and ISV identification and these will be used to genetic variability of mosquito's population assays. The molecular assays, viral isolation and genomic sequencing will be realized. In proposing this research project, we expect to answer what intrinsic and extrinsic factors, in different Aedes mosquito's populations, native from the city presenting the capacity to modulate the vector competence. These results will be associated with the genetic diversity of mosquitoes' population and the dynamic of arbovirus transmission, as an attempt to elucidate the mechanisms of gene flow and fitness that the viruses are exposed to in the vectors. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GULARTE, JULIANA SCHONS; SACCHETTO, LIVIA; DEMOLINER, MERIANE; GIRARDI, VIVIANE; DA SILVA, MARIANA SOARES; FILIPPI, MICHELI; PEREIRA, VYCTORIA MALAYHKA DE ABREU GOES; HANSEN, ALANA WITT; DA SILVA, LUANA LETICIA; FLECK, JULIANE DEISE; et al. DENV-1 genotype V linked to the 2022 dengue epidemic in Southern Brazil. Journal of Clinical Virology, v. 168, p. 8-pg., 2023-09-24. (22/03645-1)

ZINI, NATHALIA; AVILA, MATHEUS HENRIQUE TAVARES; CEZAROTTI, NATALIA MORBI; PARRA, MAISA CARLA PEREIRA; BANHO, CECILIA ARTICO; SACCHETTO, LIVIA; NEGRI, ANDREIA FRANCESLI; ARAUJO, EMERSON; BITTAR, CINTIA; MILHIN, BRUNO HENRIQUE GONCALVES DE AGUIAR; et al. Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019. PLoS Neglected Tropical Diseases, v. 18, n. 3, p. 27-pg., 2024-03-01. (22/03645-1, 13/21719-3)

BANHO, CECILIA ARTICO; MARQUES, BEATRIZ DE CARVALHO; SACCHETTO, LIVIA; LIMA, ANA KAROLINE SEPEDRO; PARRA, MAISA CARLA PEREIRA; LIMA, ALEX RANIERI JERONIMO; RIBEIRO, GABRIELA; MARTINS, ANTONIO JORGE; BARROS, CLAUDIA RENATA DOS SANTOS; ELIAS, MARIA CAROLINA; et al. Dynamic clade transitions and the influence of vaccination on the spatiotemporal circulation of SARS-CoV-2 variants. NPJ VACCINES, v. 9, n. 1, p. 12-pg., 2024-08-10. (22/03645-1, 20/10127-1, 21/11944-6, 23/14670-0, 20/04836-0)

BERNARDI, VICTORIA; SACCHETTO, LIVIA; HENDY, ADAM; FE, NELSON F.; TEIXEIRA, IGOR; MARQUES, BEATRIZ DE C.; HANLEY, KATHRYN A.; MOURAO, MARIA P. G.; LACERDA, MARCUS V. G.; VASILAKIS, NIKOS; et al. Yellow Fever Virus in Mosquitoes from Rainforest Bordering Manaus, Brazil, 2022. Emerging Infectious Diseases, v. 31, n. 4, p. 4-pg., 2025-04-01. (22/03645-1)

DA COSTA, VIVALDO G.; SAIVISH, MARIELENA V.; SINHORINI, PAOLA F.; NOGUEIRA, MAURICIO L.; RAHAL, PAULA. A meta-analysis of Chikungunya virus in neurological disorders. INFECTIOUS DISEASES NOW, v. 54, n. 5, p. 8-pg., 2024-06-25. (23/09590-7, 20/12875-5, 23/10809-3, 22/03645-1, 23/01598-9, 20/08615-8)

ESTOFOLETE, CASSIA F.; VERSIANI, ALICE F.; DOURADO, FERNANDA S.; MILHIM, BRUNO H. G. A.; PACCA, CAROLINA C.; SILVA, GISLAINE C. D.; ZINI, NATHALIA; DOS SANTOS, BARBARA F.; GANDOLFI, FLORA A.; MISTRAO, NATALIA F. B.; et al. Influence of previous Zika virus infection on acute dengue episode. PLoS Neglected Tropical Diseases, v. 17, n. 11, p. 25-pg., 2023-11-01. (22/03645-1, 22/09229-0, 18/17647-0)

TERZIAN, ANA C. B.; AZAR, SASHA R.; ESTOFOLETE, CASSIA F.; NOGUEIRA, MAURICIO L.; VASILAKIS, NIKOS. Differential Neutralization Profiles of 17DD Vaccinated Population to 17D-204 and 17DD Vaccine Strains. VACCINES, v. 12, n. 12, p. 8-pg., 2024-12-01. (22/03645-1, 22/09229-0)

RIVERA, LUIS FELIPE; LEZCANO-COBA, CARLOS; GALUE, JOSEFRANCISCO; RODRIGUEZ, XACDIEL; JUAREZ, YELISSA; DE SOUZA, WILLIAM M.; CAPITAN-BARRIOS, ZEUZ; VALDERRAMA, ANAYANSI; ABREGO, LEYDA; CEDENO, HECTOR; et al. Characteristics of Madariaga and Venezuelan Equine Encephalitis Virus Infections, Panama. Emerging Infectious Diseases, v. 30, n. 14, p. 126-pg., 2024-11-01. (22/03645-1, 18/14389-0)

SANTOS, THAYZA M. I. L.; VERSIANI, ALICE F.; CAMPOS, GUILHERME R. F.; MORAES, MARILIA M.; PARRA, MAISA C. P.; MISTRAO, NATALIA F. B.; NEGRI, ANDREIA F.; BAGNO, FLAVIA F.; GALVES, MARINA G.; MORENO, CAMILA M.; et al. Dengue and SARS-CoV-2 co-circulation and overlapping infections in hospitalized patients. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 14, p. 18-pg., 2024-11-08. (22/03645-1, 17/21452-8, 20/04836-0, 22/09229-0)

MENEZES, GABRIELA DE LIMA; SAIVISH, MARIELENA VOGEL; SACCHETTO, LIVIA; DA SILVA, GISLAINE CELESTINO DUTRA; TEIXEIRA, IGOR DA SILVA; MISTRAO, NATALIA FRANCO BUENO; NOGUEIRA, MAURICIO LACERDA; OLIVEIRA, JONAS IVAN NOBRE; BEZERRA, KATYANNA SALES; DA SILVA, ROOSEVELT ALVES; et al. Exploring Quercetin Hydrate's Potential as an Antiviral Treatment for Oropouche Virus. BIOPHYSICA, v. 3, n. 3, p. 16-pg., 2023-09-01. (22/03645-1, 20/12875-5)

PLANTE, KENNETH S.; PLANTE, JESSICA A.; AZAR, SASHA R.; SHINDE, DIVYA P.; SCHARTON, DIONNA; VERSIANI, ALICE F.; SILVA, NATALIA INGRID OLIVEIRA DA; STRANGE, TAYLOR; SACCHETTO, LIVIA; FOKAM, ERIC B.; et al. Potential of Ilheus virus to emerge. HELIYON, v. 10, n. 6, p. 15-pg., 2024-03-01. (22/03645-1)

MARQUES, RAFAEL ELIAS; SHIMIZU, JACQUELINE FARINHA; NOGUEIRA, MAURICIO LACERDA; VASILAKIS, NIKOS. Current challenges in the discovery of treatments against Mayaro fever. EXPERT OPINION ON THERAPEUTIC TARGETS, v. 28, n. 5, p. 12-pg., 2024-05-10. (22/03645-1, 21/05519-0)

SAIVISH, MARIELENA V.; NOGUEIRA, MAURICIO L.; ROSSI, SHANNAN L.; VASILAKIS, NIKOS. Exploring Iguape Virus-A Lesser-Known Orthoflavivirus. Viruses-Basel, v. 16, n. 6, p. 14-pg., 2024-06-01. (22/03645-1, 23/09590-7, 20/12875-5, 13/21719-3)

MANDOVA, TSVETELINA; SAIVISH, MARIELENA VOGEL; MENEZES, GABRIELA DE LIMA; BEZERRA, KATYANNA SALES; FULCO, UMBERTO LAINO; DA SILVA, ROOSEVELT ALVES; DA COSTA, FERNANDO BATISTA; NOGUEIRA, MAURICIO LACERDA. Antiviral Activity and Molecular Dynamics Simulation of Hops Compounds against Oropouche Virus (Peribunyaviridae). PHARMACEUTICS, v. 15, n. 12, p. 21-pg., 2023-12-01. (22/03645-1, 20/12875-5, 13/21719-3)