Abstract
Tissue Engineering employs tissue decellularization, recellularization and 3D bioprinting for production of functionalized biomaterials for transplants. Organs decellularization yields acellular biomaterials/scaffolds in which the composition and 3D structure of the extracelular matrix (ECM) are preserved. This biomaterial, when recellularized and directly applied in vivo, can provide local stimuli arise at the implantation site for stem and progenitor cells migration and proliferation, as well as cell differentiation and/or modulation of the innate imune response. 3D bioprinting may be employed to optimize the bioactivity of the decellularized scaffold both by structural customization and by association to other molecules and materials, thereby amplifying its potential applications. This Project is based on the hypothesis that decellularization, recellularization, 3D bioprinting and implantation of functionalized tissue may be utilized to better understand in vivo chemotaxis of endogenous cells towards biofunctionalized tissues to validate them for application in Veterinary Regenerative Medicine. To test this hypothesis, in vitro and in vivo models will be used. One of the wide variety of biomaterials to be employed is placental tissue, which shares with tumors several mechanisms associated with oncogenesis, such as: invasion, angiogenesis and modulation of the ECM. Therefore, the Objectives of this Project are: a) to optimize the protocol for decellularization of several tissues, aiming at the generation of scaffolds which may be associated to different cell types (recellularization) and biopolymers, yielding functionalized tissues to be utilized for organ and tissue repair and for transplant in animal models; b) to investigate the effects of placenta-derived biomaterials genesis, progression of tumor. A multidisciplinary team involving anatomists, cell biologists, surgeons and immunologists and access to state-of-the-art technologies and facilities warrant the viability of this proposal. The results obtained should contribute to better understanding not only of the cell and molecular interactions occurring at the tissue and tumor sites, but, also, allow designing novel therapeutic strategies for tissue repair and intervention in tumor growth and dissemination. (AU)
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