Development of applied multiplex electrochemical systems for detection of biomarkers related to cardiovascular diseases

• Grant number: 23/06793-4
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2024
• End date: December 31, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Bruno Campos Janegitz
• Grantee: Bruno Campos Janegitz
• Host Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil
• City of the host institution: Araras

Abstract

Multiplex electrochemical systems are technologies that allow the simultaneous detection of several biomarkers in a single biological sample, such as blood or urine, or the determination of the same compound with several electrodes, providing high data reproduction. In these systems, several electrodes can be prepared that can react in a specific way with the different biomarkers present in a specific sample, producing an electrochemical signal that can be measured and quantified. Biomarkers are substances that indicate the presence or progression of a disease. They can be proteins, enzymes, lipids, nucleic acids or metabolites, among others. The early and accurate detection of these biomarkers is essential for the diagnosis, prognosis and monitoring of several diseases, including cardiovascular ones. Cardiovascular diseases are illnesses that affect the heart and blood vessels, such as atherosclerosis, high blood pressure, acute myocardial infarction, arrhythmias and heart failure. The detection of specific biomarkers for these diseases can help in the early identification of symptoms, in the differential diagnosis and in monitoring the effectiveness of the treatment. Thus, the development of multiplex electrochemical systems for the detection of biomarkers related to cardiovascular diseases represents an important tool in diagnostic medicine, which can contribute to the prevention, treatment and control of these diseases. In this context, the present project aims at the development of platforms printed by serigraphy and 3D of multiplex systems, with up to 8 working electrodes for the detection of different analytes related to cardiovascular problems. (AU)

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, DEBORAH DE OLIVEIRA; MARINHO, FELIPE MAGALHAES; DEROCO, PATRICIA BATISTA; NEUMANN, AMANDA; CAMARGO, JESSICA ROCHA; DE FREITAS, RAFAELA CRISTINA; BERTOLIM, LUCAS VENTOSA; FATIBELLO FILHO, ORLANDO; JANEGITZ, BRUNO CAMPOS; DE OLIVEIRA, GEISER GABRIEL. A Nanodiamond-Based Electrochemical Sensor for the Determination of Paracetamol in Pharmaceutical Samples. CHEMOSENSORS, v. 12, n. 11, p. 12-pg., 2024-11-01. (19/00166-2, 23/02963-2, 23/06793-4)

CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; RODRIGUES, JESSICA DE SOUZA; DE LIMA, LUCAS FELIPE; PAIXAO, THIAGO REGIS LONGO CESAR; FRACETO, LEONARDO FERNANDES; JANEGITZ, BRUNO CAMPOS. Advancements in disposable electrochemical systems for sustainable agriculture monitoring: Trends, gaps, and applied examples. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 180, p. 14-pg., 2024-09-13. (17/21004-5, 22/03399-0, 21/10639-5, 19/04758-1, 19/23342-0, 23/12589-0, 23/00246-1, 23/06793-4, 19/23177-0, 22/00509-0, 23/00335-4)

DA SILVA, LARISSA S.; SILVA, LUIZ R. G.; PITTNER, HELOYSA S.; STEFANO, JESSICA S.; VANZIN, DOUGLAS; DORNELLAS, RAFAEL M.; JANEGITZ, BRUNO C.; DE FARIA, LUCAS V.; GALLI, ANDRESSA; ROCHA, DIEGO P.. Carbon black-integrated polylactic acid 3D-printedsensors for the voltammetric determination of pyrogallic acid: Experimental and computational insights. Electrochimica Acta, v. 541, p. 11-pg., 2025-11-20. (23/06793-4)

SILVA, LUIZ RICARDO G.; ROCHA, RAQUEL G.; ROCHA, DIEGO P.; NOSSOL, EDSON; JANEGITZ, BRUNO C.; RICHTER, EDUARDO M.; MUNOZ, RODRIGO A. A.; STEFANO, JESSICA S.. Laser-induced cobalt oxide entrapment within 3D-printed carbon electrodes for amperometric sensing. Electrochimica Acta, v. 514, p. 9-pg., 2025-01-06. (23/06793-4)

BERTOLIM, LUCAS, V; SILVA, LUIZ R. G.; STEFANO, JESSICA SANTOS; JANEGITZ, BRUNO C.. Tailoring ultra-flexibility in eco-friendly conductive filaments: The use of polypropylene in the development of a 3D-printed carbon black-based electrochemical sensor. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 996, p. 11-pg., 2025-11-01. (23/06793-4, 24/16461-1)

GERMINARE, BEATRIZ F.; CAMARGO, JESSICA R.; FERNANDES-JUNIOR, WILSON S.; JANEGITZ, BRUNO C.. Sustainable electrochemical sensor from CO2 laser pyrolyzed green leaves for carbendazim detection. Microchemical Journal, v. 218, p. 12-pg., 2025-09-06. (23/06793-4, 19/23177-0, 23/14943-6, 23/00317-6)

VON ZUBEN, THEODORA W.; CAMARGO, JESSICA R.; ROCHA, RAQUEL G.; DOS SANTOS, PAMYLA L.; RICHTER, EDUARDO M.; JANEGITZ, BRUNO C.; MUNOZ, RODRIGO A. A.; BONACIN, JULIANO A.. Advances and the growing role of additive manufacturing in the development of 3D-printed electrochemical sensors. Electrochimica Acta, v. 543, p. 30-pg., 2025-12-10. (23/06793-4, 22/00343-4, 21/05976-2, 13/22127-2, 17/11986-5)

CALDAS, NATALIA M.; BATISTA, AMANDA G.; CAMARGO, JESSICA ROCHA; AMATO, DAYENNY LOUISE D'; RONCONI, CELIA M.; SEMAAN, FELIPE S.; ROCHA, DIEGO P.; JANEGITZ, BRUNO CAMPOS; DE FARIA, LUCAS V.; DORNELLAS, RAFAEL M.. Recycling polystyrene and integrating graphite particles: A circular economy strategy for sustainable and enhanced electrochemical sensor manufacturing. Talanta, v. 298, p. 12-pg., 2026-02-01. (23/06793-4)

HENRIQUES, BRUNNA F.; NEUMANN, AMANDA; BERTOLIM, LUCAS V.; DE FREITAS, RAFAELA C.; SILVA, LUIZ R. G.; STEFANO, JESSICA S.; JANEGITZ, BRUNO C.. Development and Application of 3D-Printed Electrochemical Sensors for Ciprofloxacin Detection. Electroanalysis, v. N/A, p. 8-pg., 2024-12-26. (23/06793-4, 22/08720-1)

HENRIQUES, BRUNNA F.; NEUMANN, AMANDA; BERTOLIM, LUCAS V.; DE FREITAS, RAFAELA C.; SILVA, LUIZ R. G.; STEFANO, JESSICA S.; JANEGITZ, BRUNO C.. Development and Application of 3D-Printed Electrochemical Sensors for Ciprofloxacin Detection. Electroanalysis, v. 37, n. 1, p. 8-pg., 2025-01-01. (23/06793-4, 22/08720-1)

DE FREITAS, RAFAELA C.; CAMARGO, JESSICA ROCHA; BRAZACA, LAIS CANNIATTI; ANGNES, LUCIO; FATIBELLO-FILHO, ORLANDO; JANEGITZ, BRUNO C.. Eco-friendly screen-printed sensor using tapioca-based conductive ink modified with coconut fibers. Talanta, v. 298, p. 13-pg., 2026-09-24. (24/04116-8, 23/10141-2, 23/06793-4, 14/50867-3)

SOUZA, DANIEL C.; CARVALHO, JEFFERSON H. S.; FREITAS, RAFAELA C.; BELLETTINI, ISMAEL C.; JANEGITZ, BRUNO C.. Production of conductive microfibers based on Polybutylene adipate terephthalate and graphite using a 3D printed electrospinning system and their application as electrochemical dopamine sensor. Microchemical Journal, v. 206, p. 10-pg., 2024-08-30. (20/11336-3, 23/06793-4)

LOMBA, KALISTON A.; CARVALHO, JEFFERSON H. S.; PIMENTEL, DILTON M.; COSTA, LIVIA M. F.; ARANTES, LUCIANO C.; JANEGITZ, BRUNO C.; DOS SANTOS, WALLANS T. P.. Detection of the synthetic cathinone 4-methyl-pentedrone using lab-made screen-printed electrodes: A simple and environmentally friendly screening method for forensic applications. SENSORS AND ACTUATORS B-CHEMICAL, v. 433, p. 10-pg., 2025-06-15. (23/06793-4, 20/11336-3)

CAMARGO, JESSICA R.; CRAPNELL, ROBERT D.; BERNALTE, ELENA; CUNLIFFE, ALEXANDER J.; REDFERN, JAMES; JANEGITZ, BRUNO C.; BANKS, CRAIG E.. Conductive recycled PETg additive manufacturing filament for sterilisable electroanalytical healthcare sensors. APPLIED MATERIALS TODAY, v. 39, p. 10-pg., 2024-06-13. (23/06793-4, 23/00321-3)

ORZARI, LUIZ OTAVIO; BRAZACA, LAIS CANNIATTI; JANEGITZ, BRUNO CAMPOS. Parkinson biomarker determination with an Au microflower-enhanced electrochemical immunosensor using non-Faradaic capacitance measurements. Microchimica Acta, v. 191, n. 11, p. 13-pg., 2024-11-01. (19/23342-0, 23/10141-2, 23/06793-4)

SILVA, LUIZ R. G.; BERTOLIM, LUCAS VENTOSA; STEFANO, JESSICA S.; BONACIN, JULIANO A.; RICHTER, EDUARDO M.; MUNOZ, RODRIGO A. A.; JANEGITZ, BRUNO C.. New route for the production of lab-made composite filaments based on soybean oil, polylactic acid and carbon black nanoparticles, and its application in the additive manufacturing of electrochemical sensors. Electrochimica Acta, v. 513, p. 11-pg., 2025-02-10. (23/06793-4)

NEUMANN, AMANDA; ORZARI, LUIZ OTAVIO; BONACIN, JULIANO ALVES; JANEGITZ, BRUNO CAMPOS. Properties Evaluation of Different Graphite Brands through the Development of Electrochemical Sensors for Phenolic Compounds Detection. ACS OMEGA, v. 10, n. 6, p. 9-pg., 2025-02-04. (23/06793-4, 23/02963-2)

SILVA, LUIZ RICARDO G.; STEFANO, JESSICA S.; KALINKE, CRISTIANE; CRAPNELL, ROBERT D.; BRAZACA, LAIS C.; MARCOLINO-JUNIOR, LUIZ H.; BERGAMINI, MARCIO F.; BANKS, CRAIG E.; JANEGITZ, BRUNO C.. Dual-Target Additively Manufactured Electrochemical Sensor for the Multiplexed Detection of Protein A29 and DNA of Human Monkeypox Virus. ACS OMEGA, v. 9, n. 30, p. 12-pg., 2024-07-17. (17/21097-3, 23/06793-4)

CAMARGO, JESSICA; CRAPNELL, ROBERT D.; BERNALTE, ELENA; JANEGITZ, BRUNO C.; BANKS, CRAIG E.. Water-Based Conductive Ink for the Production of Carbon Black Screen-Printed Electrodes and the Detection of Tryptophan. ACS APPLIED ELECTRONIC MATERIALS, v. 7, n. 12, p. 12-pg., 2025-06-04. (23/06793-4, 23/00321-3, 19/23177-0)

GERMINARE, BEATRIZ F.; FERNANDES-JUNIOR, WILSON S.; CAMARGO, JESSICA R.; JANEGITZ, BRUNO C.. Cork-based electrochemical sensors obtained by laser-induced graphene: A green alternative for sodium nitrite detection in beverage samples. Microchimica Acta, v. 192, n. 9, p. 15-pg., 2025-08-21. (23/14943-6, 23/00317-6, 23/06793-4)

CLETO, SABRINA; NEUMANN, AMANDA; BERTOLIM, LUCAS V.; DE FREITAS, RAFAELA C.; CAMARGO, JESSICA R.; JANEGITZ, BRUNO C.. Treatment with CO₂ laser in SPE with Two Working Electrodes for Electrochemical Detection of Propranolol. Journal of the Electrochemical Society, v. 172, n. 8, p. 9-pg., 2025-07-01. (23/02963-2, 23/06793-4)