FUNCTIONALIZED IRON OXIDE NANOPARTICLES AS A CARRIER SYSTEM TO dsRNA PLANT DELIVERY

• Grant number: 24/22185-7
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: April 14, 2025
• End date: May 28, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Leonardo Fernandes Fraceto
• Grantee: Leonardo Fernandes Fraceto
• Visiting researcher: Humphrey Hak Ping Yiu
• Visiting researcher institution: Heriot-Watt University, Edinburgh, Scotland
• Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil
• Associated research grant: 21/10639-5 - Center for Research on Biodiversity Dynamics and Climate Change, AP.CEPID

Abstract

Nanoparticle technology has demonstrated significant potential in advancing sustainable agriculture by improving the efficiency of active ingredient (a.i.) delivery while reducing required dosages and overcoming plant barriers. Among innovative pest control methods, RNA interference (RNAi) has gained attention due to its gene silencing mechanism, which employs small interfering RNAs (siRNAs) to inhibit pathogen replication. Spray-induced gene silencing (SIGS) has emerged as a promising approach for RNAi application; however, the persistence and systemic translocation of double-stranded RNA (dsRNA) applied as a spray remain challenging, with environmental degradation limiting its effectiveness to 4-14 days. Nanocarriers offer a potential solution to protect and transport dsRNA molecules efficiently into plant tissues to address these limitations. A functionalized iron oxide nanoparticle system is a promising alternative for enhancing the delivery of dsRNA to targeted plant cells. This project aims to establish a collaborative partnership with Prof. Yiu's group at Heriot-Watt University, UK, to develop hybrid functionalized iron oxide nanoparticles for agricultural applications. Prof. Yiu brings extensive expertise in synthesizing and functionalizing magnetic nanoparticles (MNPs) using polymer coatings, enabling precise binding, tracking, and delivery of dsRNA. This collaboration will foster cross-disciplinary knowledge transfer, advancing the synthesis of hybrid MNP systems tailored for SIGS applications. The proposed research includes four months of virtual discussions and in-person visits to refine strategies and develop robust dsRNA delivery systems, strengthening sustainable agricultural practices. (AU)