Carbon nanoparticles as fluorescent probes in 3D cell bioimaging. Detection of reactive nitrogen species from novel ruthenium complexes in cytotoxicity studies.

• Grant number: 25/09760-5
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2025
• End date: October 31, 2028
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Renata Galvão de Lima
• Grantee: Renata Galvão de Lima
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Luciana Oliveira de Almeida ; Marília Cristina Oliveira Souza

Abstract

Carbon dots are carbon nanoparticles that have fluorescent properties, are biocompatible and easy to obtain. They are widely used as cell markers in bioimaging as well as in analyte sensing. Regarding 3D cells, they represent an advanced technology in cell culture that better simulates the natural environment of the body, allowing more precise studies of cell biology, development of therapies and drug testing. The combination of carbon dots and 3D cells can be used to mark or track 3D cells, facilitating the visualization and analysis of complex biological processes. In the meantime, the objective of this project is to mark three-dimensional cells with fluorescent carbon dots based on polycarboxylate polymers to detect reactive nitrogen species, produced by new ruthenium nitrosyl complexes from redutimetric or photochemical stimulation. This combination is a very innovative approach and can open many doors in cell therapy, diagnosis and understanding of cellular mechanisms. (AU)