Innovative Instrumentation and Methodological Advances in Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

• Grant number: 25/20625-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2026
• End date: January 31, 2029
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Claudimir Lucio Do Lago
• Grantee: Claudimir Lucio Do Lago
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo

Abstract

Capillary electrophoresis (CE) is an analytical separation technique with high efficiency that, despite its potential, has not achieved the expected popularity compared to techniques such as high-performance liquid chromatography (HPLC). Although well-established in biochemical niches, its broader adoption is limited by challenges such as reproducibility, instrumental cost, and operational complexity. This project aims to help overcome these barriers through innovations in instrumentation and methodologies, focusing on contactless capacitively coupled conductivity detection (C4D) and novel approaches using thermal markers, charge-based electropherograms, mobility spectra, and indirect calibration. This project seeks to redefine the paradigm of capillary electrophoresis by addressing historical limitations through innovative technical solutions and strategic collaborations. The outcomes have the potential to revitalize the technique, expanding its use beyond specialized niches and establishing it as a viable alternative in chemical analysis. (AU)