Transport and Energetic Metabolism in Biological Systems

• Grant number: 94/03581-1
• Support Opportunities: Research Projects - Thematic Grants
• Start date: September 01, 1995
• End date: September 30, 1999
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Paulo Arruda
• Grantee: Paulo Arruda
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant(s): 97/10689-1 - "somaclonal variation-induced aluminum sensitive mutant from an aluminum-tolerant maize inbred line. inheritance..."., PUB.ART
• Associated scholarship(s): 98/00565-6 - Expression of the plant uncoupling mitochondrial protein (pump) in yeast., BP.PD

Abstract

The main goals of this project is to study at biochemical and molecular level, the structure-function in plants and mammals of the following proteins involved in transport and energetic metabolism: the Na+/H+ transporter, the plasma membranes H+-ATPase, the organic acid transporters and the hexoquinase. These proteins are involved in key steps in metabolic transport and energy regeneration in the cells. Many of than are encoded by multigene families which could be related to function or adaptation to different environment. The specific goals of the project will be: a) a cloning and characterization of the HE-1 gene of pig and maize; b) cloning and characterization of members of the multigene family of plasma membrane H+-ATPase from maize; c) expression of H+-ATPase in yeast to study the kinetic properties of the enzyme; d) study of the role of H+-ATPase on the mechanism of aluminum tolerance and organic acid transport; e) identification of a plant organic acid transporter; f) cloning of the hexoquinase gene from maize. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAIA‚ I.G.; BENEDETTI‚ C.E.; LEITE‚ A.; TURCINELLI‚ S.R.; VERCESI‚ A.E.; ARRUDA‚ P.. AtPUMP: an Arabidopsis gene encoding a plant uncoupling mitochondrial protein1. FEBS Letters, v. 429, n. 3, p. 403-406, 1998. (94/03581-1)