Structural and functional analysis of multi-prosthetic systems for the pathogenicity of Xanthomonas axonopodis pv. citri

• Grant number: 05/59243-3
• Support Opportunities: Research Projects - Thematic Grants
• Start date: July 01, 2006
• End date: November 30, 2011
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Shaker Chuck Farah
• Grantee: Shaker Chuck Farah
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 08/00669-0 - Structural and biochemical essays of XAC3230, a putative virulence factor of NAD: arginin ADP-ribosyltransferase family of phytophatogen Xanthomonas axonopodis pv citri, BP.IC

Abstract

In this project we propose to study some specific molecular systems that contribute to the pathogenicity of Xanthomonas axonopodis pv. citri (Xac), the cause of citrus canker disease. We plan to use biochemical, spectroscopic and genetic techniques, in combination with the production of recombinant proteins to study the type III (T3SS) and type IV (T4SS) secretion systems and quorum sensing signal transduction pathway in Xac. We also plan to employ structural biology techniques to determine the structures of subunits and subunit complexes of the T3SS, T4SS and quorum sensing pathway. As well as providing details regarding the molecular mechanisms used by this phytopathogen to cause disease, these structures promise to reveal details regarding the function of orthologous systems found in other pathogens, including human pathogens with significant impact on public health. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORREA, FERNANDO; SALINAS, ROBERTO KOPKE; BONVIN, ALEXANDRE M. J. J.; FARAH, CHUCK S.. Deciphering the role of the electrostatic interactions in the alpha-tropomyosin head-to-tail complex. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, v. 73, n. 4, p. 902-917, Dec. 2008. (05/59243-3)

SOUZA, DIORGE P.; ANDRADE, MAXUEL O.; ALVAREZ-MARTINEZ, CRISTINA E.; ARANTES, GUILHERME M.; FARAH, CHUCK S.; SALINAS, ROBERTO K.. A Component of the Xanthomonadaceae Type IV Secretion System Combines a VirB7 Motif with a N0 Domain Found in Outer Membrane Transport Proteins. PLOS PATHOGENS, v. 7, n. 5, p. e1002031, 2011. (05/59243-3)

SOUZA, DIORGE P.; OKA, GABRIEL U.; ALVAREZ-MARTINEZ, CRISTINA E.; BISSON-FILHO, ALEXANDRE W.; DUNGER, GERMAN; HOBEIKA, LISE; CAVALCANTE, NAYARA S.; ALEGRIA, MARCOS C.; BARBOSA, LEANDRO R. S.; SALINAS, ROBERTO K.; et al. Bacterial killing via a type IV secretion system. NATURE COMMUNICATIONS, v. 6, MAR 2015. (05/59243-3, 09/54009-3, 11/07777-5)

CAPPELLETTI, PAOLA A.; DOS SANTOS, RAFAEL FREITAS; DO AMARAL, ALEXANDRE M.; HOMEM, RAFAEL AUGUSTO; SOUZA, THAIS DOS SANTOS; MACHADO, MARCOS A.; FARAH, CHUCK S.. Structure-Function Analysis of the HrpB2-HrcU Interaction in the Xanthomonas citri Type III Secretion System. PLoS One, v. 6, n. 3, p. e17614, 2011. (05/59243-3)

DUNGER, GERMAN; GAROFALO, CECILIA G.; GOTTIG, NATALIA; GARAVAGLIA, BETIANA S.; PEREDA ROSA, MARIA C.; FARAH, CHUCK S.; ORELLANO, ELENA G.; OTTADO, JORGELINA. Analysis of three Xanthomonas axonopodis pv. citri effector proteins in pathogenicity and their interactions with host plant proteins. MOLECULAR PLANT PATHOLOGY, v. 13, n. 8, p. 865-876, OCT 2012. (05/59243-3)