Understanding the metabolism of lysine in cereals

• Grant number: 04/16039-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2005
• End date: April 30, 2010
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Principal Investigator: Ricardo Antunes de Azevedo
• Grantee: Ricardo Antunes de Azevedo
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais: Cláudia de Mattos Bellato ; Lyndel Wayne Meinhardt

Abstract

The main idea for this project comes from the years of development and involvement associated with the member of this group. During these years, there has been an orderly natural sequence of development in the research accomplished by this group; however, there still remain a number of key gaps and unanswered questions that demand a large focused project such as the one being proposed. This area of research is extremely interesting because of the fact that cereals (the main source of nutrients in the human diet) are poor sources of quality nutrition. This lack of quality is the direct result of the low quantity of lysine and other essential amino acids (for example tryptophan) that are found in the seeds and especially in the endosperm. In general, the project involves the study of the storage proteins of sorghum, maize and barley as well as the characterization of the enzymes that control the metabolism of lysine in maize mutants that are considered to have high lysine levels. The main objectives are to obtain more data regarding lysine metabolism and storage proteins of the different cereals, which will contribute to the future genetic improvements and manipulations, required to increase the protein nutrition of this group of plants. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VENDEMIATTI, ARIANE; FERREIRA, RENATO RODRIGUES; GOMES, LUIZ HUMBERTO; MEDICI, LEONARDO OLIVEIRA; AZEVEDO, RICARDO ANTUNES. Nutritional Quality of Sorghum Seeds: Storage Proteins and Amino Acids. Food Biotechnology, v. 22, n. 4, p. 377-397, 2008. (04/16039-4)

ALBERTO MOLDES, CARLOS; MANUEL CAMINA, JOSE; MEDICI, LEONARDO OLIVEIRA; TSAI, SIU MUI; AZEVEDO, RICARDO ANTUNES. Physiological effects of glyphosate over amino acid profile in conventional and transgenic soybean (Glycine max). PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, v. 102, n. 2, p. 134-141, FEB 2012. (04/16039-4)

FERREIRA, R. R.; MEINHARDT, L. W.; AZEVEDO, R. A.. Lysine and threonine biosynthesis in sorghum seeds: characterisation of aspartate kinase and homoserine dehydrogenase isoenzymes. Annals of Applied Biology, v. 149, n. 1, p. 77-86, Aug. 2006. (04/16039-4)

VARISI, VANDERLEI A.; MEDICI, LEONARDO O.; VAN DER MEER, INGRID; LEA, PETER J.; AZEVEDO, RICARDO A.. Dihydrodipicolinate synthase in opaque and floury maize mutants. Plant Science, v. 173, n. 4, p. 458-467, Oct. 2007. (04/16039-4)

VARISI, VANDERLEI A.; CAMARGOS, LILIANE S.; AGUIAR, LEANDRO F.; CHRISTOFOLETI, RENATA M.; MEDICI, LEONARDO O.; AZEVEDO, RICARDO A.. Lysine biosynthesis and nitrogen metabolism in quinoa (Chenopodium quinoa): study of enzymes and nitrogen-containing compounds. Plant Physiology and Biochemistry, v. 46, n. 1, p. 11-18, Jan. 2008. (04/16039-4)

MEDICI, LEONARDO OLIVEIRA; GAZIOLA, SALETE APARECIDA; VARISI, VANDERLEI APARECIDO; CARMEZINI DE PAULA, JOSE ANTONIO; FERREIRA, RENATO RODRIGUES; AZEVEDO, RICARDO ANTUNES. DIALLELIC ANALYSIS FOR LYSINE AND OIL CONTENTS IN MAIZE GRAINS. CIENTIA AGRICOL, v. 66, n. 2, p. 6-pg., 2009-01-01. (04/16039-4)

MEDICI, LEONARDO OLIVEIRA; GAZIOLA, SALETE APARECIDA; VARISI, VANDERLEI APARECIDO; CARMEZINI DE PAULA, JOSE ANTONIO; FERREIRA, RENATO RODRIGUES; AZEVEDO, RICARDO ANTUNES. Análise dialélica para conteúdos de lisina e óleo em grãos de milho. Scientia Agricola, v. 66, n. 2, p. 204-209, 2009. (04/16039-4)

SCHMIDT, DAIANA; RIZZI, VANESSA; GAZIOLA, SALETE A.; MEDICI, LEONARDO O.; VINCZE, EVA; KOZAK, MARCIN; LEA, PETER J.; AZEVEDO, RICARDO A.. Lysine metabolism in antisense C-hordein barley grains. Plant Physiology and Biochemistry, v. 87, p. 73-83, FEB 2015. (11/21123-8, 04/16039-4)

POMPEU, GEÓRGIA BERTONI; VENDEMIATTI, ARIANE; GRATÃO, PRISCILA LUPINO; GAZIOLA, SALETE APARECIDA; LEA, PETER JOHN; AZEVEDO, RICARDO ANTUNES. Saccharopine dehydrogenase activity in the high-lysine opaque and floury maize mutants. Food Biotechnology, v. 20, n. 1, p. 55-64, Jan. 2006. (04/16039-4)

LEONARDO OLIVEIRA MEDICI; SALETE APARECIDA GAZIOLA; VANDERLEI APARECIDO VARISI; JOSÉ ANTONIO CARMEZINI DE PAULA; RENATO RODRIGUES FERREIRA; RICARDO ANTUNES AZEVEDO. Análise dialélica para conteúdos de lisina e óleo em grãos de milho. Scientia Agricola, v. 66, n. 2, p. 204-209, 2009-04-00. (04/16039-4)