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Models to estimate amino acids requirements for broilers, laying type pullets and laying hens

Abstract

The aim of this project is to elaborate models to estimate amino acids requirements for broilers, laying type pullets and laying hens, based on factorial method, which consisted on partitioning the requirements into maintenance, growing and/or production. Firstly, the method to determine maintenance amino acid requirement based on nitrogen balance technique will be standardized. After that, six metabolic assays with roosters will be conducted to determine lysine, methionine and threonine maintenance requirements. A serie of 12 dose-response assays with broiler chickens will be conducted to determine the best lysine, methionine and threonine levels and their efficiencies of utilization for body protein deposition in each phase (1 to 7; 8 to 21; 22 to 34 and 35 to 42 days of age). Another serie of 12 dose-response assays will be conducted with laying type birds from I1to 6 weeks, 7 to 12 weeks and 13 to 18 weeks and 24 to 40 weeks to determine the best lysine, methionine and threonine levels based on performance results and efficiencies of amino acid utilization. For each assay, a basal diet will be formulated according to nutritional requirements for each phase, deficient in the amino acid evaluated. The crystalline amino acid will be supplemented in the basal diet in order to get amino acid levels from 70 to 110% of requirements. The efficiencies of amino acid utilization in broilers and laying type pullets will be determined based on linear regression of body protein and amino acid deposition as function of amino acid intake, considering the slope of regression. The best amino acid level will be determined based on polynomial regression, linear response plateau and exponential models. The amino acid requirements for growing and egg production will be calculated considering the protein or amino acid deposition on body or egg and efficiencies of amino acid utilization. The models to estimate amino acid requirements will be elaborated based on maintenance, growing and egg production requirements determined in this study. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA, E. P.; LIMA, M. B.; SAKOMURA, N. K.; MORAES, L. E.; PERUZZI, N. J.. Weight gain responses of laying-type pullets to methionine plus cystine intake. ANIMAL, v. 14, n. 2, SI, p. S294-S302, . (08/50557-3)
SARCINELLI, M. F.; SAKOMURA, N. K.; DORIGAM, J. C. P.; SILVA, E. P.; VENTURINI, K. S.; LIMA, M. B.; GONCALVES, C. A.. Modelling Japanese quail responses to methionine plus cystine, threonine and tryptophan intake. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 263, . (08/50557-3)
DA SILVA, EDNEY PEREIRA; SAKOMURA, NILVA KAZUE; SARCINELLI, MYRIELLE FREIRE; DE PAULA DORIGAM, JULIANO CESAR; VENTURINI, KATIANI SILVA; DE LIMA, MICHELE BERNARDINO. Modeling the response of Japanese quail hens to lysine intake. LIVESTOCK SCIENCE, v. 224, p. 69-74, . (08/50557-3)
SILVA, E. P.; MALHEIROS, E. B.; SAKOMURA, N. K.; VENTURINI, K. S.; HAUSCHILD, L.; DORIGAM, J. C. P.; FERNANDES, J. B. K.. Lysine requirements of laying hens. LIVESTOCK SCIENCE, v. 173, p. 69-77, . (08/50557-3)
SILVA, E. P.; SAKOMURA, N. K.; DORIGAM, J. C. P.; LIMA, M. B.; MALHEIROS, E. B.; FERNANDES, J. B. K.. Sulfur amino acid requirements for pullets in growth and pre-laying trials. ANIMAL PRODUCTION SCIENCE, v. 61, n. 15, . (08/50557-3)

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