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Estimation of ideal essential amino acid ratio for poultry by deletion method

Grant number: 13/07136-5
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Nilva Kazue Sakomura
Grantee:Juliano César de Paula Dorigam
Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated research grant:08/50557-3 - Models to estimate amino acids requirements for broilers, laying type pullets and laying hens, AP.TEM


The objective of this research is to determine the ideal profile of essential amino acids (AA) for breeders and broilers of Cobb 500 genotype using the deletion method. The essential amino acids (EAA) that will be studied are lysine (1), methionine + cystine (2), threonine (3), tryptophan (4), isoleucine (5), leucine (6), histidine (7), phenylalanine +tyrosine (8), glycine + serine (9), valine (10) and arginine (11). The trials with broilers will be conducted in three periods (6-21, 22-37 and 38 to 53 days) with 72 birds each. It will be 12 treatments and six replicates of one bird each. The trials will last 15 days and will be obtained nitrogen intake (NI) and nitrogen deposition (ND) in the nitrogen balance trials. In the breeders trial 96 breeder hens will be used with 24 weeks of age. There will be 12 treatments and eight replicates of one bird each. The NI and ND will be collected at 10 weeks after diet adaptation. For broiler and breeder hens, the diets will be obtained similarly. The 12 treatments will be a control diet (CP) and 11 diets 30% deficient in each EAA. The CP diet will meet the ideal ratio of Brazilian tables. The CP diet will be diluted with corn starch to meet 70% of the requirements and the other treatments will be achieved by supplementation of industrial AA in the deficient diets, except the EAA to be tested. In each treatment it will be calculated the efficiency of utilization of the EAA and the ideal ratio will be obtained by dividing the efficiencies. In the third test will be used 132 roosters with 22 treatments and six replicates of one bird each. Within five days of testing will be collected NI and ND. The treatments will be 11 diets meeting the requirements of the EAA for maintenance, except the AA to be studied (reduced 50%) at two levels of NI (0.25 and 0.50 g N/PCkg0.75/d). The diets will be supllied by precision feeding techique. For each EAA will be traced a straight line and the maintenance requirement will be determined by the amount to maintain nitrogen balance. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE PAULA DORIGAM, JULIANO CESAR; SAKOMURA, NILVA KAZUE; SOARES, LETICIA; KOCHENBORGER FERNANDES, JOAO BATISTA; SUENDER, ANGELA; LIEBERT, FRANK. Modelling of lysine requirement in broiler breeder hens based on daily nitrogen retention and efficiency of dietary lysine utilization. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 226, p. 29-38, . (13/07136-5)
DORIGAM, J. C. P.; SAKOMURA, N. K.; SARCINELLI, M. F.; GONCALVES, C. A.; DE LIMA, M. B.; PERUZZI, N. J.. Optimal in-feed amino acid ratio for broiler breeder hens based on deletion studies. JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, v. 101, n. 6, p. 1194-1204, . (13/07136-5)
DORIGAM, J. C. P.; SAKOMURA, N. K.; DE LIMA, M. B.; SARCINELLI, M. F.; SUZUKI, R. M.. Establishing an essential amino acid profile for maintenance in poultry using deletion method. JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, v. 100, n. 5, p. 884-892, . (13/07136-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DORIGAM, Juliano César de Paula. The ideal essential amino acid ratio for maintenance, growth and production of poultry. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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