Cellular antioxidant systems response to mitochondrial oxidative stress induced by...
| Grant number: | 98/13012-5 |
| Support type: | Research Projects - Thematic Grants |
| Duration: | April 01, 1999 - December 31, 2003 |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Principal Investigator: | Aníbal Eugênio Vercesi |
| Grantee: | Aníbal Eugênio Vercesi |
| Home Institution: | Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated grant(s): | 02/00552-9 - Petr Jezek | Inst Physiology - Czech Academy of Sciences - República Tcheca,
AV.EXT 01/04438-3 - Hagai Rottenberg | Hahnemann University School of Medicine - United States, AV.EXT |
Abstract
Up to 2% of the oxygen consumed by the mitochondrial respiratory chain suffers monoeletronic reduction, mostly at the level of the semiquinone form of coenzyme Q, leading to the generation of the superoxide radical, which can in turn produce other reactive oxygen species, such as hydrogen peroxide and the hydroxyl radical. Under situations in which mitochondrial generation of reactive oxygen species is increased, or the mitochondrial antioxidant defense mechanisms are impared, these reactive oxygen species may accumulate and lead to irreversible damage of mitochondrial DNA, membrane lipids and proteins, resulting in mitochondrial dysfunction and consequent cell death. In this project, we propose to study the mechanisms by which Ca2+ stimulates mitochondrial reactive oxygen species generation, detailing the consequences of these species on different mitochondrial components. We also plan to investigate new mechanisms by which cells defend themselves against mitochondrial oxidative stress and drugs that may prevent mitochondrial injury induced by reactive oxygen species in isolated mitochondria, intact cells, tissues and organs in vivo. In addition, we propose to study calcium homeostasis and mitochondrial bioenergetics in emergent pathogenic fungi with the aim to better understand their physiology and may contribute to the development of more effective chemotherapy against these opportunistic pathogens. (AU)