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Novel analytical strategies for studies involving biomolecules

Grant number: 06/07309-3
Support type:Research Projects - Thematic Grants
Duration: August 01, 2007 - July 31, 2011
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Elias Ayres Guidetti Zagatto
Grantee:Elias Ayres Guidetti Zagatto
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Co-Principal Investigators:Marco Aurelio Zezzi Arruda ; Maria Fernanda Georgina Gine Rosias ; Ronei Jesus Poppi
Assoc. researchers:José Alberto Fracassi da Silva ; Lauro Tatsuo Kubota ; Ljubica Tasic ; Maria Fernanda Georgina Gine Rosias
Associated scholarship(s):09/11584-8 - Implementation of flow/sequential injection analysis (FIA/SIA) workstations projected for sample preparation and bidimensional separations management, BP.PD
08/03981-4 - Comparative metallomics of transgenic and non-transgenic soybeans (Glicine max l. Merril)using bidimensional separation systems, BP.DR


Novel analytical strategies involving bio-molecules, such as e.g. enzymes, metalloenzymes and metalloproteins will be implemented, with emphasis to use of biomarkers for evaluating tolerance to oxidative stress. Analytical procedures will be developed for the determination of anti-oxidants, markers of oxidative damage, metals and proteins; novel non-destructive methodologies for fast in-situ analysis in biological materials presenting high heterogeneity in structure and high variability in shape. The feasibility of exploiting isotope fractioning for evaluation of biochemical processes involving metals and biomolecules will be investigated. Furthermore, multi-pumping flow systems will be designed for direct analyses of the protein spots as well as for implementing mini-columns of biomaterials for pesticide determination. The acquired knowledge will be disseminated and the project development will be evaluated and re-directioned through workshops involving all the participants. (AU)

Scientific publications (14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARNEIRO, RENATO LAJARIM; POPPI, RONEI JESUS. Infrared imaging spectroscopy and chemometric tools for in situ analysis of an imiquimod pharmaceutical preparation presented as cream. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 118, p. 215-220, JAN 24 2014. Web of Science Citations: 11.
CARNEIRO, RENATO LAJARIM; POPPI, RONEI JESUS. Homogeneity study of ointment dosage forms by infrared imaging spectroscopy. Journal of Pharmaceutical and Biomedical Analysis, v. 58, p. 42-48, JAN 25 2012. Web of Science Citations: 7.
SABIN, GUILHERME P.; BREITKREITZ, MARCIA C.; DE SOUZA, ANDRE M.; DA FONSECA, PATRICIA; CALEFE, LUPERCIO; MOFFA, MARIO; POPPI, RONEI J. Analysis of pharmaceutical pellets: An approach using near-infrared chemical imaging. Analytica Chimica Acta, v. 706, n. 1, p. 113-119, NOV 7 2011. Web of Science Citations: 23.
SABIN, GUILHERME POST; DE CARVALHO ROCHA, WERICKSON FORTUNATO; POPPI, RONEI JESUS. Study of the similarity between distribution maps of concentration in near-infrared spectroscopy chemical imaging obtained by different multivariate calibration approaches. Microchemical Journal, v. 99, n. 2, p. 542-547, NOV 2011. Web of Science Citations: 7.
SILVA, CLAUDINEIA R.; BARROS, VALDEMIR A. F.; BASSO, LUIZ C.; ZAGATTO, ELIAS A. G. Spectrophotometric flow injection monitoring of sulfide during sugar fermentation. Talanta, v. 85, n. 3, p. 1703-1705, SEP 15 2011. Web of Science Citations: 4.
BAPTISTAO, MARIANA; DE CARVALHO ROCHA, WERICKSON FORTUNATO; POPPI, RONEI JESUS. Quality control of the paracetamol drug by chemometrics and imaging spectroscopy in the near infrared region. Journal of Molecular Structure, v. 1002, n. 1-3, p. 167-171, SEP 14 2011. Web of Science Citations: 10.
VIDA, ANA C. F.; SASAKI, MILTON K.; GOMES, TACIANA F.; SILVA, CLAUDINEIA R.; FERES, MARIO A.; ZAGATTO, E. A. G. Zone trapping/merging zones in flow analysis: A novel approach for rapid assays involving relatively slow chemical reactions. Talanta, v. 85, n. 1, p. 259-263, JUL 15 2011. Web of Science Citations: 6.
CARLOS, CAMILA; MARETTO, DANILO A.; POPPI, RONEI J.; SATO, MARIA INES Z.; OTTOBONI, LAURA M. M. Fourier transform infrared microspectroscopy as a bacterial source tracking tool to discriminate fecal E. coli strains. Microchemical Journal, v. 99, n. 1, p. 15-19, 2011. Web of Science Citations: 9.
ROCHA‚ W.F.C.; POPPI‚ R.J. Multivariate control charts based on net analyte signal (NAS) and Raman spectroscopy for quality control of carbamazepine. Analytica Chimica Acta, v. 705, n. 1, p. 35-40, 2011.
FORTES, PAULA R.; FERES, MARIO A.; ZAGATTO, ELIAS A. G.; LIMA, JOSE L. F. C. Sequential injections as an alternative to gradient exploitation for implementing differential kinetic analysis in a flow injection system. Talanta, v. 81, n. 4-5, p. 1409-1412, JUN 15 2010. Web of Science Citations: 7.
FRACASSI DA SILVA, JOSE ALBERTO; DE CASTRO, NATHAN VITORAZZI; DE JESUS, DOSIL PEREIRA; FARIA, ADRIANA F.; DE SOUZA, MARCUS V. N.; LEAL DE OLIVEIRA, MARCONE AUGUSTO. Fast determination of ethambutol in pharmaceutical formulations using capillary electrophoresis with capacitively coupled contactless conductivity detection. ELECTROPHORESIS, v. 31, n. 3, p. 570-574, FEB 2010. Web of Science Citations: 14.
VALDERRAMA, PATRICIA; POPPI, RONEI JESUS. Determination of propranolol enantiomers in plasma and urine by spectrofluorimetry and second-order standard addition method. Analytica Chimica Acta, v. 651, n. 1, p. 31-35, SEP 28 2009. Web of Science Citations: 26.
DIAS, A. C. B.; FIGUEIREDO, E. C.; GRASSI, V.; ZAGATTO, E. A. G.; ARRUDA, M. A. Z. Molecularly imprinted polymer as a solid phase extractor in flow analysis. Talanta, v. 76, n. 5, p. 988-996, SEP 15 2008. Web of Science Citations: 64.
FERES‚ M.A.; FORTES‚ P.R.; ZAGATTO‚ E.A.G.; SANTOS‚ J.L.M.; LIMA‚ J.L.F.C. Multi-commutation in flow analysis: Recent developments and applications. Analytica Chimica Acta, v. 618, n. 1, p. 1-17, 2008.

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