Synthesis and studies of peptides and analogues involved principally in the renin-angiotensin and kallikrein-kinin system

• Grant number: 07/56480-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2008
• End date: February 29, 2012
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: clovis ryuichi nakaie
• Grantee: clovis ryuichi nakaie
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Antonio de Miranda ; Ismael Dale Cotrim Guerreiro da Silva ; Shirley Schreier ; Suma Imura Shimuta
• Associated scholarship(s): 10/11671-5 - Interaction of antimicrobial peptides with model membranes., BP.DR; 10/03658-9 - THE ROLE OF ANGIOTENSIN II AND ANGIOTENSIN-(1-7) IN PROLIFERATION AND PROGRESSION OF BREAST CANCER, BP.MS; 09/08336-2 - Role of the extracellular and transmembrane domains to binding and activation of kinin B1 receptor: correlation with the structure-activity of the angiotensin II type I receptor., BP.DR

Abstract

Since we belong to one of the few research groups that not only synthesizes peptides, but has also endeavored to refine this methodology, we have been able to acquire in the last decades, a good level of autonomy in obtaining this relevant class of macromolecules. Peptide analogues have been produced by different strategies, including the use of paramagnetic aminoacids, allowing us good lines of diversification in our research. Based on these considerations, we decided in the present project, to propose a broader line of research and integrated by ten sub-items, weighted principally towards the important physiological systems, renin-angiotensin and kallikrein-kinin. Some peptides present in these systems such as angiotensins, bradykinin and others, together with their analogues containing various alterations, principally of the non-natural type, in their structures, will be examined. The receptors involved in these systems will also be evaluated in different ways, ranging from experiments of site-directed mutations to studies of the difficulties of synthesis, purification and conformational analysis of some of their transmembranar hydrophobic fragments. In addition to classic investigation of the structure-function type, others such as the effect of radiations and of proteolytic enzymes on their structures, as well as the possibility of participation in tumoral processes, will also involve these selected peptides. Complementing these essentially more biological studies, other investigations apparently dissociated from the central theme will also be developed, since all will be inserted, in one way or another, into our continual effort to learn the Chemistry and Physico-Chemistry of peptides. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA MOLINA WOLGIEN, MARIA DEL CARMEN; COTRIM GUERREIRO DA SILVA, ISMAEL DALE; PINTO NAZARIO, AFONSO CELSO; NAKAIE, CLOVIS RIUCHE; ALVES CORREA-NORONHA, SILVANA APARECIDA; RIBEIRO DE NORONHA, SAMUEL MARCOS; FACINA, GIL. Genetic Association Study of Angiotensin II Receptor Types 1 (A168G) and 2 (T1247G and A5235G) Polymorphisms in Breast Carcinoma among Brazilian Women. BREAST CARE, v. 9, n. 3, p. 176-181, 2014. (07/56480-0)

MARTIN, RENAN PAULO; FILIPPELLI-SILVA, RAFAEL; SHUKLA, AK. Non-radioactive binding assay for bradykinin and angiotensin receptors. G PROTEIN-COUPLED RECEPTORS, PT B, v. 149, p. 9-pg., 2019-01-01. (07/56480-0, 11/02208-2, 14/20965-3)

MARTIN, RENAN P.; FILIPPELLI-SILVA, RAFAEL; RODRIGUES, ELIETE S.; NAKAIE, CLOVIS R.; SHIMUTA, SUMA I.. A fluorimetric binding assay for angiotensin II and kinin receptors. JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS, v. 79, p. 55-59, MAY-JUN 2016. (07/56480-0, 11/02208-2, 14/20965-3)

JOAO P. F. VIEIRA; ERICK F. POLETTI; RENATA F. F. VIEIRA; VINICIUS VEREDAS; CESAR C. SANTANA; CLOVIS R. NAKAIE. Alternative and simple normal-phase HPLC enantioseparation of a chiral amino acid-type spin label derivative. Journal of the Brazilian Chemical Society, v. 24, n. 11, p. 1840-1845, 2013-11-00. (07/56480-0)

BARROS, ALEXANDRE J.; ITO, CHRISTIAN M.; MAKINO, ELAINE N.; CEMBRANELLI, FERNANDO A. M.; MORAES, FRANCISCO C.; SOUZA, SINVAL E. G.; OLIVEIRA, LAERTE; SHIMUTA, SUMA I.; NAKAIE, CLOVIS R.. Factors regulating tachyphylaxis triggered by N-terminal-modified angiotensin II analogs. Biological Chemistry, v. 390, n. 12, p. 1265-1270, DEC 2009. (07/56480-0)