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Molecular epidemiology and integrated management of Huanglongbing (asiaticus and americanus) in the State of São Paulo


This research project has ten subprojects. Its joint analysis will allow a better understanding .f the structure and behavior of the Huanglongbing path system in São Paulo, a sine qua non condition for the definition of a more rational disease management. The proposed subprojects are: 1) development, evaluation, and application of real time PCR for diagnosis and detection of Ca. Liberibacter asiaticus and Ca. L. americanus in plants and insect vectors and in the study of the vector (Diaphorina citri) infectivity; 2) Spatial and temporal molecular epidemiology of HLB (asiaticus and americanus) in greenhouse conditions considering two different inoculums sources; 3) spatial and temporal analysis of HLB epidemics in commercial orchards; 4) determination of acquisition and inoculation periods of Ca. Liberibacter americanus e Ca. L. asiaticus for Diaphorina citri; 5) determination of incubation and latent periods of HLB (asiaticus and americanus) after transmission by Diaphorina citri; 6) effect of temperature and geographic region on the progress of HLB (asiaticus and americanus); 7) geographic distribution of Ca. Liberibacter americanus e Ca. L. asiaticus in the state of São Paulo; 8) progress of colonization of HLB (asiaticus and americanus) in different citrus varieties with different ages; 9) determination of natural infectivity of the vector (Diaphorina citri) in orchards with different HLB (asiaticus and americanus) incidences; 10) strategies of control of HLB in commercial orchards based on more favorable times for elimination of symptomatic plants and vector control. The core of the project is the HLB epidemiology, always considering both, Ca. L. americanus e Ca. L. asiaticus. All subprojects will be assessed through visual inspection and molecular methods. These will be applied for the first time in the epidemiological study of HLB. The final objective is to control the disease in a more rational way. Many of the proposed studies are original for Ca. L. asiaticus. All of them are original for Ca. L. americanus, the HLB form most disseminated in São Paulo. (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Avispas sueltas en los naranjales 
Let loose the wasps on the orange groves 
Fighting the yellow dragon 

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASSANEZI, RENATO B.; PRIMIANO, V, ISABELA; MOREIRA, ALECIO S. Inoculum reduction and vector control on the temporal progress of citrus variegated chlorosis incidence. Pest Management Science, APR 2021. Web of Science Citations: 0.
WULFF, NELSON ARNO; FASSINI, CAMILA GIACOMO; MARQUES, VIVIANI VIEIRA; MARTINS, ELAINE CRISTINA; BONONI COLETTI, DANIELA APARECIDA; TEIXEIRA, DIVA DO CARMO; SANCHES, MARCIO MARTINELLO; BOVE, JOSEPH MARIE. Molecular Characterization and Detection of 16SrIII Group Phytoplasma Associated with Huanglongbing Symptoms. PHYTOPATHOLOGY, v. 109, n. 3, p. 366-374, MAR 2019. Web of Science Citations: 1.
BASSANEZI, R. B.; BELASQUE, JR., J.; MONTESINO, L. H. Frequency of symptomatic trees removal in small citrus blocks on citrus huanglongbing epidemics. CROP PROTECTION, v. 52, p. 72-77, OCT 2013. Web of Science Citations: 13.
BASSANEZI, RENATO B.; MONTESINO, LUIZ H.; GIMENES-FERNANDES, NELSON; YAMAMOTO, PEDRO T.; GOTTWALD, TIM R.; AMORIM, LILIAN; BERGAMIN FILHO, ARMANDO. Efficacy of Area-Wide Inoculum Reduction and Vector Control on Temporal Progress of Huanglongbing in Young Sweet Orange Plantings. PLANT DISEASE, v. 97, n. 6, p. 789-796, JUN 2013. Web of Science Citations: 51.
LOPES, S. A.; FRARE, G. F.; CAMARGO, L. E. A.; WULFF, N. A.; TEIXEIRA, D. C.; BASSANEZI, R. B.; BEATTIE, G. A. C.; AYRES, A. J. Liberibacters associated with orange jasmine in Brazil: incidence in urban areas and relatedness to citrus liberibacters. PLANT PATHOLOGY, v. 59, n. 6, p. 1044-1053, DEC 2010. Web of Science Citations: 16.
LOPES, S. A.; FRARE, G. F.; BERTOLINI, E.; CAMBRA, M.; FERNANDES, N. G.; AYRES, A. J.; MARIN, D. R.; BOVE, J. M. Liberibacters Associated with Citrus Huanglongbing in Brazil: `Candidatus Liberibacter asiaticus' Is Heat Tolerant, `Ca. L. americanus' Is Heat Sensitive. PLANT DISEASE, v. 93, n. 3, p. 257-262, MAR 2009. Web of Science Citations: 59.

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