Molecular epidemiology and integrated management of Huanglongbing (asiaticus and americanus) in the State of São Paulo

• Grant number: 07/55013-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: February 01, 2008
• End date: March 31, 2012
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Armando Bergamin Filho
• Grantee: Armando Bergamin Filho
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Pesquisadores principais:( Últimos ): Renato Beozzo Bassanezi
• Pesquisadores principais:( Antigos ): Lilian Amorim

Abstract

This research project has ten subprojects. Its joint analysis will allow a better understanding .f the structure and behavior of the Huanglongbing path system in São Paulo, a sine qua non condition for the definition of a more rational disease management. The proposed subprojects are: 1) development, evaluation, and application of real time PCR for diagnosis and detection of Ca. Liberibacter asiaticus and Ca. L. americanus in plants and insect vectors and in the study of the vector (Diaphorina citri) infectivity; 2) Spatial and temporal molecular epidemiology of HLB (asiaticus and americanus) in greenhouse conditions considering two different inoculums sources; 3) spatial and temporal analysis of HLB epidemics in commercial orchards; 4) determination of acquisition and inoculation periods of Ca. Liberibacter americanus e Ca. L. asiaticus for Diaphorina citri; 5) determination of incubation and latent periods of HLB (asiaticus and americanus) after transmission by Diaphorina citri; 6) effect of temperature and geographic region on the progress of HLB (asiaticus and americanus); 7) geographic distribution of Ca. Liberibacter americanus e Ca. L. asiaticus in the state of São Paulo; 8) progress of colonization of HLB (asiaticus and americanus) in different citrus varieties with different ages; 9) determination of natural infectivity of the vector (Diaphorina citri) in orchards with different HLB (asiaticus and americanus) incidences; 10) strategies of control of HLB in commercial orchards based on more favorable times for elimination of symptomatic plants and vector control. The core of the project is the HLB epidemiology, always considering both, Ca. L. americanus e Ca. L. asiaticus. All subprojects will be assessed through visual inspection and molecular methods. These will be applied for the first time in the epidemiological study of HLB. The final objective is to control the disease in a more rational way. Many of the proposed studies are original for Ca. L. asiaticus. All of them are original for Ca. L. americanus, the HLB form most disseminated in São Paulo. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BASSANEZI, RENATO BEOZZO; PRIMIANO, ISABELA VESCOVE. Huanglongbing and Citrus Variegated Chlorosis Integrated Management Based on Favorable Periods for Vector Population Increase and Symptom Expression. PLANT DISEASE, v. 105, n. 10, p. 3037-3047, OCT 2021. (07/55013-9)

BASSANEZI, RENATO B.; MONTESINO, LUIZ H.; GIMENES-FERNANDES, NELSON; YAMAMOTO, PEDRO T.; GOTTWALD, TIM R.; AMORIM, LILIAN; BERGAMIN FILHO, ARMANDO. Efficacy of Area-Wide Inoculum Reduction and Vector Control on Temporal Progress of Huanglongbing in Young Sweet Orange Plantings. PLANT DISEASE, v. 97, n. 6, p. 789-796, JUN 2013. (07/55013-9, 05/00718-2)

LOPES, S. A.; FRARE, G. F.; CAMARGO, L. E. A.; WULFF, N. A.; TEIXEIRA, D. C.; BASSANEZI, R. B.; BEATTIE, G. A. C.; AYRES, A. J.. Liberibacters associated with orange jasmine in Brazil: incidence in urban areas and relatedness to citrus liberibacters. PLANT PATHOLOGY, v. 59, n. 6, p. 1044-1053, DEC 2010. (07/55013-9, 05/00718-2)

WULFF, NELSON ARNO; FASSINI, CAMILA GIACOMO; MARQUES, VIVIANI VIEIRA; MARTINS, ELAINE CRISTINA; BONONI COLETTI, DANIELA APARECIDA; TEIXEIRA, DIVA DO CARMO; SANCHES, MARCIO MARTINELLO; BOVE, JOSEPH MARIE. Molecular Characterization and Detection of 16SrIII Group Phytoplasma Associated with Huanglongbing Symptoms. PHYTOPATHOLOGY, v. 109, n. 3, p. 366-374, MAR 2019. (07/55013-9)

DE GODOY GASPAROTO, MARIA CANDIDA; PRIMIANO, ISABELA VESCOVE; BASSANEZI, RENATO B.; LOURENCO, SILVIA AFONSECA; MONTESINO, LUIZ H.; WULFF, NELSON ARNO; MARTINS, ELAINE CRISTINA; BERGAMIN FILHO, ARMANDO; AMORIM, LILIAN. revalent Transmission of `Candidatus Liberibacter asiaticus' over `Ca. Liberibacter americanus' in a Long-Term Controlled Environmen. PHYTOPATHOLOGY, v. 112, n. 1, p. 180-188, JAN 2022. (07/55013-9)

BASSANEZI, RENATO B.; PRIMIANO, V, ISABELA; MOREIRA, ALECIO S.. Inoculum reduction and vector control on the temporal progress of citrus variegated chlorosis incidence. Pest Management Science, v. 77, n. 7, p. 3333-3340, JUL 2021. (05/00718-2, 07/55013-9)

LOPES, S. A.; FRARE, G. F.; BERTOLINI, E.; CAMBRA, M.; FERNANDES, N. G.; AYRES, A. J.; MARIN, D. R.; BOVE, J. M.. Liberibacters Associated with Citrus Huanglongbing in Brazil: `Candidatus Liberibacter asiaticus' Is Heat Tolerant, `Ca. L. americanus' Is Heat Sensitive. PLANT DISEASE, v. 93, n. 3, p. 257-262, MAR 2009. (07/55013-9)

BASSANEZI, R. B.; BELASQUE, JR., J.; MONTESINO, L. H.. Frequency of symptomatic trees removal in small citrus blocks on citrus huanglongbing epidemics. CROP PROTECTION, v. 52, p. 72-77, OCT 2013. (07/55013-9, 05/00718-2)

LOPES, S. A.; FRARE, G. F.; BERTOLINI, E.; CAMBRA, M.; FERNANDES, N. G.; AYRES, A. J.; MARIN, D. R.; BOVE, J. M.. Liberibacters Associated with Citrus Huanglongbing in Brazil: 'Candidatus Liberibacter asiaticus' Is Heat Tolerant, 'Ca. L. americanus' Is Heat Sensitive. PLANT DISEASE, v. 93, n. 3, p. 6-pg., 2009-03-01. (07/55013-9)