Clinical, homogasometric and oxidative stress evaluation of normal and pneumonic sheep

• Grant number: 10/00044-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2010
• End date: February 29, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Roberto Calderon Gonçalves
• Grantee: Roberto Calderon Gonçalves
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The occurrence of pneumonia is common in sheep and usually cause a reduction in the production and economic losses. In domestic species, pneumonia is accompanied by intense inflammatory response and accumulation of phagocytic cells in the lungs, causing structural damage of the respiratory tract due to oxidative stress resulting from the release of large amounts of Reactive oxygen species (EROs) during the respiratory burst. This work aims to study the behavior of ROS and oxidative stress during the inflammatory response in pneumonia in normal and pneumonic sheep and its effects on lung function. Will be used 40 sheep, 20 clinically healthy and 20 with pneumonia. The animals will be subjected to clinical examination and divided into two groups: i) G1/control group - clinically healthy animals and; ii) G2 - animals with pneumonia. The generation of EROs and occurrence of oxidative stress will be determined indirectly by measuring the enzymatic activity of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) and concentrations of Total Glutathione (GSH-t) and Thiobarbituric Acid (TBARS) in peripheral blood by colorimetric method. The inflammatory response will be evaluated by hemogram and total protein and plasma fibrinogen, and lung function by determining the blood gas parameters Oxygen Pressure (PO2) Pressure of Carbon Dioxide (PCO2), Pressure Hydrogen (pH), Oxygen Saturation (SO2) , Bicarbonate (HCO3-), Total Carbon Dioxide (TCO2) and Base Excess (EB) in arterial blood. (AU)