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Semen freezeability of ram supplemented with vitamin A or submitted to low intensity laser terapy

Grant number: 13/13438-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2013
Effective date (End): July 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Eneiva Carla Carvalho Celeghini
Grantee:Tamie Guibu de Almeida
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The frozen ram semen has been object of large studies, considering that, his using makes possible the use of artificial insemination (AI) on sheep although, the pregnancy rate achieved through IA has still been short, probably, dues to the necessity of spermatozoids thawed overcome the cervical barrier which opens opportunity of searching for greater knowledge of the semen to be frozen. Supplementation with vitamin A helps in renovation of seminiferous epithelium and decreasing of concentration of reactive oxygen species; on the other hand, the use of low intensity laserterapia (LT), which encourages the cellular mitosis, could also serve as an aid in the proliferation of seminiferous epithelium and thus contributing to obtain better indices in freezing semen. This project aims to analyze the ram semen freezability, submitted or not to testicular degeneration, supplemented with vitamin A or low intensity lasertherapy. Eighteen sheep will be used and distributed into six treatments groups: 1) control: no degenerated and no treated (n=3), 2) no degenerated and supplemented with vitamin A (n=3), 3) no degenerated and submitted to lasertherapy (n=3), 4) degenerated and no treated (n=3), 5) degenerated and supplemented with vitamin A (n=3), 6)degenerated and submitted to low intensity lasertherapy (n=3). During the experimental period weekly assessment will be made including: reproductive exam, evaluation of testicular and rectal temperature. Soon, after harvest, semen will be evaluated increased by extender base of egg, packaged in plastic straws and cryopreserved, using an automated system, being kept in cryogenic cylinders (-196º C). Then, the semen straw will be thawed (37ºC/30 seconds) and evaluated for motility (CASA), sperm morphology (DIC), integrity of plasma and acrosomal membranes, mitochondrial function and oxidative stress. The data will be submitted to analysis of variance and the averages compared by Fisher's test employing the statistical program, Statistical Analysis System (SAS Institute Inc., 2004).

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISSACO, LEONARDO; DE ARRUDA, RUBENS PAES; RODRIGUES ALVES, MAIRA BIANCHI; TORRES, MARIANA ANDRADE; LEMES, KLEBER MENEGON; PRADO-FILHO, ROBERTO ROMANO; DE ALMEIDA, TAMIE GUIBU; CESAR DE ANDRADE, ANDRE FURUGEN; CARVALHO CELEGHINI, ENEIVA CARLA. Cholesterol-loaded cyclodextrin is efficient in preserving sperm quality of cryopreserved ram semen with low freezability. REPRODUCTIVE BIOLOGY, v. 20, n. 1, p. 14-24, MAR 2020. Web of Science Citations: 1.
DE ALMEIDA, TAMIE GUIBU; RODRIGUES ALVES, MAIRA BIANCHI; BATISSACO, LEONARDO; TORRES, MARIANA ANDRADE; CESAR DE ANDRADE, ANDRE FURUGEN; MINGOTI, RODOLFO DANIEL; DE ARRUDA, RUBENS PAES; CARVALHO CELEGHINI, ENEIVA CARLA. Does low-level laser therapy on degenerated ovine testes improve post-thawed sperm characteristics?. Lasers in Medical Science, v. 34, n. 5, p. 1001-1009, JUL 2019. Web of Science Citations: 0.

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