Experimental systemic candemia in immunocompetent (BALB / c) and immunodeficient (BALB / c - SCID) mice by Candida tropicalis ATCC 66,029: Immunodepression influence systemic in the candidemia by Candida tropicalis

• Grant number: 08/11379-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2009
• End date: September 30, 2011
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Claudete Rodrigues Paula Gandra
• Grantee: Claudete Rodrigues Paula Gandra
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The state of immunosuppression fosters the proliferation of hematologic Candida species, including non-albicans species of the patient's own microbiota, causing serious injuries to the already delicate situation. This situation causes serious deterioration of the delicate framework of these patients, increase the risk of death and costs spent during treatment. The experimental study in immunocompromised animals inoculated with an emerging species will allow understand how it develops this infection in these patients, forming scientific basis for future experiments related to virulence factors, therapies and preventive procedures. In our search for candidemia experimental system will be inoculated from the side towards the tail of the animal 200 µL of a suspension containing (3 x 105) of viable cells of Candida tropicalis and analyzes the pathogenesis of this model will be evaluated through the study of survival, curve of weight, histological and microbiological testing, but also through the study of immunohistochemistry, in the proposed model. At the end of the study hope to better understand the effect of immunosuppression in candidaemia by Candida tropicalis in this model. (AU)