Molecular characterization of human polyomavirus JC from patients with AIDS, with and without progressive multifocal leukoencephalopathy in São Paulo, Brazil

• Grant number: 07/04681-1
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2008 - March 31, 2010
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Claudio Sergio Pannuti
• Grantee: Claudio Sergio Pannuti
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

JC virus (JCV) is the etiologic agent of progressive multifocal leukoencephalopathy (PML), a disease of the central nervous system of immunocompromised patients manifested by focal lesions without mass effect. The aim of the present study is to compare the prevalence of different genotypes of JCV in cerebrospinal fluid (CSF) and peripheral blood samples from patients with AIDS and PML and in blood samples from patients with AIDS without PML, matched with PML cases by CD4+ cells number. Samples will be obtained from AIDS patients admitted to three medical institutions (Instituto de Infectologia Emílio Ribas, Hospital das Clínicas da FMUSP e UNICAMP). Additionally, amplification and sequencing of the regulatory region of the virus strains obtained from the two groups of patients will be carried out. Detection of JC virus will be performed by two different PCR techniques (conventional and real-time PCR). In JCV positive samples the viral load will be determined by real-time PCR and molecular genotyping will be carried out by amplification and sequencing of the VP1 region of the virus genome. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA NALI, LUIZ HENRIQUE; CENTRONE, CRISTIANE DE CAMPOS; PALMA URBANO, PAULO ROBERTO; PENALVA-DE-OLIVEIRA, AUGUSTO CESAR; VIDAL, JOSE ERNESTO; MIRANDA, ERIQUE PEIXOTO; PANNUTI, CLAUDIO SERGIO; DOMINGUES DA SILVA FINK, MARIA CRISTINA. HIGH PREVALENCE OF THE SIMULTANEOUS EXCRETION OF POLYOMAVIRUSES JC AND BK IN THE URINE OF HIV-INFECTED PATIENTS WITHOUT NEUROLOGICAL SYMPTOMS IN SAO PAULO, BRAZIL. Revista do Instituto de Medicina Tropical de São Paulo, v. 54, n. 4, p. 201-205, JUL-AUG 2012. (07/04681-1)

DOMINGUES FINK, MARIA CRISTINA; PENALVA DE OLIVEIRA, AUGUSTO CESAR; ROMANO, CAMILA MALTA; VIDAL, JOSE ERNESTO; PALMA URBANO, PAULO ROBERTO; TATENO, ADRIANA FUMIE; OLIVEIRA, CRISTINA MENDES; DE ALBUQUERQUE LUNA, EXPEDITO JOSE; PANNUTI, CLAUDIO SERGIO. Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy. Journal of Clinical Virology, v. 48, n. 1, p. 6-10, MAY 2010. (07/04681-1)