Effect of materials used as pit and fissures sealants in the in situ dental biofilm

• Grant number: 08/06576-3
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2008 - October 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Denise Pedrini
• Grantee: Denise Pedrini
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Dental biofilm accumulation in surfaces is one of the factors of caries and other dental diseases development. It is established that different types of dental materials release several substances, which exert an antibacterial action against oral microorganisms in vitro and in vivo. The inorganic composition of the dental biofilm is relevant to dental caries, as reserves of fluoride, calcium and inorganic phosphorus contributing to the saturation of fluid biofilm in relation to dental enamel when the pH decreases due to carbohydrates fermentation. Moreover, the concentration of inorganic components in biofilm has an inverse relationship to the development of caries. Thus, it would be important to evaluate whether pit and fissures sealants would have a significant action on dental biofilm that could influence the bacterial effects on enamel. Therefore, the objective of this study will be to verify the effect of materials used as sealant of pit and fissures in acidogenicity, in fluoride, calcium, phosphorus and alkali-soluble carbohydrates and composition of dental biofilm formed in situ. Ten volunteers will take part in this double-blind study, using 3 materials, (Fuji IX GP, AuraLay XF e Aegis) and a control group (enamel slab). Four experimental phases with 7 days each will be established, during wich the volunteers will wear acryclic palatal appliances containing 4 specimens of each material or enamel slabs covered by gauze for dental biofilm accumulation. A washout period of 7 days will be allowed between each phase. Ten days before and during the whole experiment the volunteers will use a non-fluoridated dentifrice and will receive instructions on oral hygiene. In each phase, 6 times/day, volunteers will remove the appliance from the oral cavity and will drop two 20% sucrose solution onto each specimen and control group. The acidogenicity of dental biofilm assessment formed in two areas (mesial and distal) of the slots acrylic palatal appliances which contains specimens or enamel slabs be evaluated on the 7th day. The initial pH will be measured and after that sucrose 20% will be dropped in each specimen and in the control group and new pH measurements will be done after 5, 15 and 30 minutes to calculate the pH variation. In the 8th day the biofilm will be collected for analysis of total anaerobic microorganisms, total streptococci and mutans streptococci. The concentration of fluoride, calcium, phosphorus and alkali-soluble carbohydrates of dental biofilm will also be analyzed. The results will be analyzed considering the different materials and the variables: pH variation, the concentrations of fluoride, calcium, phosphorus and alkali-soluble carbohydrates and composition of dental biofilm. The data will be submitted to a test of normality and homogeneity and then the appropriate statistical test will be applied. The significance limit will be set at 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, LILIAN; PEDRINI, DENISE; OKAMOTO, ANA CLAUDIA; JARDIM JUNIOR, ELERSON GAETTI; HENRIQUES, TASSIA ARAUJO; CANNON, MARK; BOTAZZO DELBEM, ALBERTO CARLOS. Biochemical and microbiological characteristics of in situ biofilm formed on materials containing fluoride or amorphous calcium phosphate. AMERICAN JOURNAL OF DENTISTRY, v. 26, n. 4, p. 207-213, AUG 2013. (08/02084-9, 08/06576-3)