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Comparative study between standard culture in selective media and Real Time Polymerase Chain Reaction assay for detection of maternal colonization by group B Streptococcus

Abstract

Group B Streptococcus (GBS) or Streptococcus agalactiae emerged in the 1970s as the leading cause of neonatal morbidity and mortality. Today, GBS remains one of the leading causes of sepsis and meningitis in newborns, despite important prevention efforts. The human gastrointestinal tract is the natural reservoir for GBS and is the likely source of vaginal colonization. GBS disease in newborns usually results from ascending spread of GBS into the amniotic fluid, which leads to neonatal colonization and to invasive disease in some infants. Preterm infants have the higher neonatal mortality rates. In 2002, the Centers for Disease Control and Prevention (CDC) guidelines suggest that the diagnosis of GBS colonization in pregnant women is best made via culture of a combined rectal and vaginal swab using a selective enrichment (Todd-Hewitt) broth at 35 to 37 week's gestation. However, the sensitivity of cultures in detecting GBS colonization varies and results take up/36 hours. The rapid and highly sensitive real-time polymerase chain reaction assay for the specific detection of GBS from vaginorectal samples, obtained from pregnant women, approved by the US Food and Drug Administration (FDA), provides improvements in the accuracy and rapidity of GBS colonization screening compared to the standard culture-based method using the recommended selective enrichment broth. The main purpose of this study is to evaluate the performance of a real-time PCR assay, developed "in-house", to detect antenatal GBS colonization in pregnant women at 35 to 37 weeks of gestation, and in pregnancies complicated by preterm labor and preterm premature rupture of membranes, comparing it with the standard culture method for screening for GBS carriage. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAULA DURANTE ANDRADE; JOICE DE SOUZA RUSSO; JÉSSICA BALIERO GOUVEIA; CLÁUDIA RAQUEL CANTARELLI COSTA; KETTI GLEYZER OLIVEIRA; MICHELLI GIANETTI; EMANUEL BORGES VÍTOR ANJOS; TYCHA BIANCA SABAINI PAVAN; MARIANA FURQUIM DA SILVA MARTINS; JOSIELE FRANCO; MARIA LAURA COSTA; MARCELO LUÍS NOMURA; CARLOS EMÍLIO LEVY; RENATO PASSINI JÚNIOR; SANDRA CECILIA BOTELHO COSTA. Molecular Characterization of Group B Streptococcus Serotypes By Multiplex Polymerase Chain Reaction. MedicalExpress (São Paulo, online), v. 4, n. 4, p. -, Ago. 2017.

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