Antimicrobials residues and occurrence of mycotic mastitis in dairy cattle

• Grant number: 09/07377-7
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2009
• End date: September 30, 2011
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Juliana Rodrigues Pozzi Arcaro
• Grantee: Juliana Rodrigues Pozzi Arcaro
• Host Institution: Instituto de Zootecnia. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Nova Odessa , SP, Brazil

Abstract

The Bovine mastitis is an inflammation of the mammary gland often caused by infectious agents that causes economic losses to nationally and internationally livestock milk. The use of practices to reduce the duration and occurrence of new infections has been constant objective of the producers. Among them, administration of antibiotics for prophylaxis and treatment of dairy herds in the milk can generate waste compromising product quality and safety of food. The prolonged and indiscriminate use of antibiotics leads to selection of resistant bacteria, cause allergies in susceptible individuals and may encourage agents opportunistic infections, including yeast and filamentous fungi. This project aims to detect the general presence of antimicrobial residues in milk and study its relationship with occurrence of mycotic mastitis in dairy cows. Samples of milk will be collected from cooling tanks, balloons meters and breast quarters of all cows in lactation (400) properties of milk from four types B and C of the region of Campinas, fortnightly, during period of 60 days. The animals will be evaluated for the presence of clinical mastitis with test Tamis, presence of subclinical mastitis by the California Mastitis Test (CMT) and somatic cell count (SCC). For the detection of residues of antibiotics will be used commercial test ((Delvotestâ SP). The microbiological analysis shall be conducted through the media culture in blood agar, Mac Conkey agar and Sabouraud with chloramphenicol. The dilution technique is used for counting of CFU / ml of yeast and filamentous fungi. The data will be analyzed by chi-square test for qualitative verification of the associations and Person's correlation tests to identify the correlation between quantitative variables. (AU)