Study of cellular mechanisms involved in photodynamic therapy

• Grant number: 10/00488-5
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2010
• End date: March 31, 2012
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Juliana Ferreira Strixino
• Grantee: Juliana Ferreira Strixino
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: Leandro José Raniero

Abstract

The concept of Photodynamic Therapy (PDT) is cytotoxicity photoinduction of cells proliferating involving a photosensitizer agent (PS), light source and molecular oxygen. PDT is technique that induces damage to the tumor tissue and is the administration of a photosensitive drug that is selectively retained in neoplastic tissue and produces singlet oxygen when irradiated with a wavelength appropriate in the presence of molecular oxygen. This study used the PS will be the photogem®, a hematoporphyrin derivative produced in Russia and being used in PDT in Brazil and the PDZ®, PS-based chlorine produced in Russia. The PFs will be administered in animals intraperitoneally at doses of 12 mg / kg (HPD) and 8 mg / kg (PDZ). The light source chosen is (Brazil) and used for HPD laser diode at 630 and for chlorin 660 nm QuantumTech intensity of 200 mW/cm and 300 J/cm light dose and the intensity of a PDZ 100 mW/cm and 100 J/cm. This study aims at establishing a basis for diagnosis and therapy, studying the cellular mechanisms of PDT for the combination of the techniques of FT-Raman, electron microscopy, immunohistochemistry, and molecular markers, comparing cellular mechanisms of PDT-induced different photosensitizers and correlating with cellular resistance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, J. C.; FERREIRA-STRIXINO, J.; FONTANA, L. C.; PAULA, L. M.; RANIERO, L.; MARTIN, A. A.; CANEVARI, R. A.. Apoptosis-associated genes related to photodynamic therapy in breast carcinomas. Lasers in Medical Science, v. 29, n. 4, p. 1429-1436, JUL 2014. (10/00488-5, 11/09523-0, 09/18440-1)