Experimental controlled and randomized study in rabbits about local toxicity of octal-2-cianoacrilate tissue adhesive when used in stabilization of cartilage grafts

• Grant number: 10/10971-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2010
• End date: September 30, 2012
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: José Eduardo Lutaif Dolci
• Grantee: José Eduardo Lutaif Dolci
• Host Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP). Fundação Arnaldo Vieira de Carvalho. São Paulo , SP, Brazil

Abstract

Grafts of cartilages are used broadly in otorhinolaringology surgery. Their main functions include filling of an area with deformity and aid in the support estructures. Classicaly, they are fixed by suture. However, the growing interest in outpatient procedures brings the need of faster and feasible fixation methods with minimal inlflammatory reaction. The tissue adhesives simplify complex surgical procedures. They can be defined as any substance that keeps the union among two or more tissues. Favorable characteristics of a surgical adhesive include (1) good adhesion strength, (2) biocompatibility, (3) biodegradable properties, (4) low tissue reactivity and (5) accessible cost. Cianoacrylate derived are among the most largely used. Nevertheless, their local tissue toxicity in slightly vascularized surfaces, such as the interface between two cartilages, is not yet well defined, limiting their use. Objective: To determine the local tissue reaction of octyl-2-cyanoacrilate adhesive when used in stabilization of cartilages grafts, in rabbits. Materials and method: A total of 18 Male New Zealand rabbits, weighing between 2 and 3 Kg, will be used in this study. After intravenous anesthesia, a rectangular piece of cartilage will be removed from the left ear, measuring 1,0 cm by 1,5 cm, which will be grafted in the cartilage of external right ear. The animals will be divided into two groups: adhesive group, graft fixation with glue; and control group, graft placed without glue. The inflammatory tissue reaction to the graft will be checked, in 2, 6 and 12 weeks, using a graduated scale based on the inflammatory cell infiltrate population (neutrophils, lymphocytes and foreign-body giant cells): 0, none to minimal; 1, mild; 2, moderate; and 3, severe; and its extent (focal, patchy or diffuse). (AU)