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New methodological proposal for the immobilization of cells of Candida guilliermondii in pva-cryogel for bioproduction of xylitol


At GPF/FAENQUIL work on immobilized cells has been outstanding in recent years and huge advances have been made with these systems. At the moment the studies and advances point to new strategies for techniques of immobilization and support materials. So the present work seeks to determine suitable conditions for the immobilization of the Candida guilliermondii FTI 20037 yeast in PVAcryogel for the production of xylitol, as well evaluating the behavior of the biocatalyst/support system in the system of repeated shaking in Erlenmeyers flasks and in a mixing bioreactor. It is hoped with the results obtained, to have mastery of a methodology for the immobilization of cells of C. guilliermondii in PVA-cryogel, that will permit the production of xylitol in mixing bioreactor. It should be pointed out that this work forms part of the line of research “Biotechnological exploitation of sugarcane bagasse for the production of xylitol through immobilized cells” created with support from FAPESP. (AU)

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