Biotechnological exploitation of Antarctic-derived fungi

• Grant number: 10/17033-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2011
• End date: May 31, 2013
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lara Durães Sette
• Grantee: Lara Durães Sette
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• City of the host institution: Rio Claro
• Associated researchers: Adalberto Pessoa Junior ; André Rodrigues ; Ângelo Samir Melim Miguel ; Eleni Gomes ; Fernando Carlos Pagnocca ; Rafaella Costa Bonugli Santos

Abstract

The Antarctic continent is characterized by restrictive environmental conditions of climate, habitat and biogeography. The main organisms adapted to the harsh conditions of this environment are the micro-organisms, which have potential application in several biotechnological processes. Among the enzymatic arsenal of fungi stand out ligninases, cellulases, xylanases, lipases and proteases that have wide application in various sectors of socio-economic importance. The present work aims to use the 350 fungi (~250 filamentous fungi and 100 yeasts) maintained in the research collection of Microbial Resources Division (CPQBA / UNICAMP), which were recently isolated from samples of terrestrial and marine Antarctic ecosystems in order to: a) select fungi able to produce environmental and/or industrial enzymes; b) produce and optimize the production of such enzymes by the selected fungi; c) characterize biochemically the enzymes highly produced; d) detect and characterize the genes that code for the production of the highly produced enzymes; e) taxonomically identify fungi that have potential for biotechnological applications and; f) deposit the isolates identified in the Collection of Antarctic Micro-organisms structured within the scope of Brazilian Collection of Microorganisms Environment and Industry - CBMAI (CPQBA / UNICAMP). It is worth to mention that new yeast strains are being obtained from four different samples of marine sediments, which will also be prospected and studied in this work. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, n. 2, p. 215-225, DEC 17 2015. (10/17033-0, 13/19486-0, 10/08352-5)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, SI, p. 874-885, OCT 2016. (10/17033-0, 13/19486-0, 10/08352-5)

DUARTE, A. W. F.; DAYO-OWOYEMI, I.; NOBRE, F. S.; PAGNOCCA, F. C.; CHAUD, L. C. S.; PESSOA, A.; FELIPE, M. G. A.; SETTE, L. D.. Taxonomic assessment and enzymes production by yeasts isolated from marine and terrestrial Antarctic samples. EXTREMOPHILES, v. 17, n. 6, p. 1023-1035, NOV 2013. (10/17033-0, 10/08352-5)

DOS SANTOS, JULIANA A.; VIEIRA, JULIANA M. F.; VIDEIRA, ALEXANDRE; MEIRELLES, LUCAS A.; RODRIGUES, ANDRE; TANIWAKI, MARTA H.; SETTE, LARA D.. Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production. AMB EXPRESS, v. 6, MAR 24 2016. (11/16765-0, 10/17033-0, 13/19486-0, 13/08338-0)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, p. 11-pg., 2015-12-17. (13/19486-0, 10/08352-5, 10/17033-0)

NASCIMENTO, TALITA C. E. S.; DUTRA MOLINO, JOAO VITOR; DONADO, PRISCILA R. S.; MONTALVO, GUALBERTO S. A.; DOS SANTOS, WELLINGTON L.; GOMES, JOSE ERICK G.; SANTOS, JOAO H. P. M.; DA SILVA, ROBERTO; SETTE, LARA DURAES; PESSOA JUNIOR, ADALBERTO; et al. Antarctic fungus proteases generate bioactive peptides from caseinate. Food Research International, v. 139, JAN 2021. (10/17033-0)

MACHADO, SUELLEN; FEITOSA, VALKER; PILLACA-PULLO, OMAR; LARIO, LUCIANA; SETTE, LARA; PESSOA JR, ADALBERTO; ALVES, HARLEY. Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor. BIOENGINEERING-BASEL, v. 9, n. 11, p. 13-pg., 2022-11-01. (10/17033-0, 12/23726-4, 13/19486-0, 16/07957-7)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, p. 12-pg., 2016-10-01. (13/19486-0, 10/08352-5, 10/17033-0)