Metabolic effects and high density lipoprotein (HDL) functions in diabetes type 2 patients with and without obstructive coronary desease

• Grant number: 10/51826-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2011
• End date: January 31, 2013
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Whady Armindo Hueb
• Grantee: Whady Armindo Hueb
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Coronary artery disease is responsible for an 80% mortality rate among diabetes mellitus type 2 patients. The cause for this high mortality rate is still not well understood. DM2 affects lipid metabolism, which causes dyslipidemia, characterized by high trygliceryde levels and low HDL-cholesterol levels. It also affects lipid transfer proteins, such as CETP and PLTP. HDL has an important anti-aterogenic role in serum and in cholesterol reverse transport and lipid transfers affect HDL formation and metabolism. It is therefore of major importance to understand HDL alterations in DM2 that can cause the loss of HDL's protective role against CAD development. The objective of this work is to investigate if lipid transfer to HDL of the four major lipids in its composition is altered in DM2 and if possible alterations are related to CAD development or anti-CAD protection in these patients. 80 DM2 CAD patients, 80 DM2 non-CAD patients, 80 non-diabetics CAD patients and 80 non-diabetics non-CAD patients will be studied. In the present study we will determine the transfer of the four major lipids to HDL by incubating radioactively labeled lipid nanoemulsions with plasma. Other factors involved in lipid transfer such as CETP, PLTP, LCAT concentrations and HDL and its subfraction composition will also be determined. The outcome of this investigation may help to explain how HDL metabolism is altered by DM2 and how these alterations can affect CAD development in those patients with DM2. (AU)