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Effects of physical exercise on insulin/IGF-1 signaling pathway in brain of diabetic rats

Abstract

Diabetes Mellitus is a chronic disease that has been associated with memory loss, neurological disorders and Alzheimer's disease (AD). Some studies show the importance of the physical exercise to prevent and minimize various neurological disorders. It is believed that the positive effects of exercise on brain function are mediated by insulin and IGF-1. However, there are few studies investigating the effects of physical training on the insulin and IGF-1 signaling pathway of diabetic animals. The main aim of the present study is to verify the role of swimming training on signaling pathway of insulin and IGF-1 in diabetic rats. Wistar rats were divided in sedentary control (SC), trained control (TC), sedentary diabetic (SD), and trained diabetic (TD). Diabetes was induced by Alloxan (32 mg kg(-1) b.w.). Training program consisted in swimming 5 days/week, 1 h/day, 6 weeks, supporting a load corresponding to 90% of maximal lactate steady state (MLSS). At the end of the training period, rats will be sacrificed and sample blood will be collected for determinations of serum glucose, insulin, GH, and IGF-1. Hippocampus and cerebellum will be extracted to determinate protein content and activity by immunoprecipitation and immunoblotting (IR, IGF-1R, IRS-1, IRS-2, PI3-q, Akt, ERK1/2, GSK-3- ², tau protein, APP and ²-Amyloide protein). All dependent variables will be analyzed by two-way analysis of variance (ANOVA) with significance level of 5%. The Bonferroni test will be used for post hoc comparisons. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORGES, MARIANA EIRAS; RIBEIRO, ALESSANDRA MUSSI; PAULI, JOSE RODRIGO; ARANTES, LUCIANA MENDONCA; LUCIANO, ELIETE; DE MOURA, LEANDRO PEREIRA; CURIACOS DE ALMEIDA LEME, JOSE ALEXANDRE; MEDEIROS, ALESSANDRA; BERTOLINI, NATALIA OLIVEIRA; SIBUYA, CLARICE YOSHIKO; GOMES, RICARDO JOSE. Cerebellar Insulin/IGF-1 signaling in diabetic rats: Effects of exercise training. Neuroscience Letters, v. 639, p. 157-161, FEB 3 2017. Web of Science Citations: 1.
DIEGUES, JOAO CARLOS; PAULI, JOSE RODRIGO; LUCIANO, ELIETE; CURIACOS DE ALMEIDA LEME, JOSE ALEXANDRE; DE MOURA, LEANDRO PEREIRA; DALIA, RODRIGO AUGUSTO; DE ARAUJO, MICHEL BARBOSA; SIBUYA, CLARICE YOSHIKO; ROSTOM DE MELLO, MARIA ALICE; GOMES, RICARDO JOSE. Spatial memory in sedentary and trained diabetic rats: Molecular mechanisms. HIPPOCAMPUS, v. 24, n. 6, p. 703-711, JUN 2014. Web of Science Citations: 16.

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