Histopathologic evaluation of the treatment of squamous cell carcinoma in mice using photodynamic therapy mediated by methylene blue

• Grant number: 10/52675-3
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2011
• End date: August 31, 2013
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: José Roberto Machado Cunha da Silva
• Grantee: José Roberto Machado Cunha da Silva
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Photodynamic Therapy (PDT) is a clinical alternative to treat a variety malignancy. This therapy uses photosensitizing molecules (PS) in the target tissue followed by irradiation with light. The principle of PDT is to generate reactive oxygen species (ROS) and/or singlet oxygen (1O2) that are capable of causing the death of the tumor. Death mechanisms used by PDT depend on the type and dose of PS, the type of light source and the irradiation dose and type of tumor. Methylene Blue (MB) is a PS can generate high concentrations of 1O2, because it has good absorption of photons in the red spectrum of visible light (>630 nm), ideal to reach the therapeutic window (600-800 nm) and have photodynamic effect. Besides the direct killing of tumor cells by apoptosis or necrosis, PDT may act indirectly causing the destruction of tumor vasculature and activation of immune response. Squamous cell carcinoma (SCC) is a type of skin cancer common in both humans and animals. The experimental model in vivo using chemical carcinogens (DMBA/TPA) mimics the conditions of histopathologic and molecular SCC developed naturally. Therefore, the objective of this project is to evaluate the morphological and molecular treatment of SCC developed experimentally in the skin of Swiss mice using the PDT mediated by methylene blue. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, ANA PAULA; NEVES, CAMILA LIMA; SILVA, ELIZANGELA DOS ANJOS; LIMA PORTELA, TANIA CRISTINA; IUNES, RENATA STECCA; COGLIATI, BRUNO; SEVERINO, DIVINOMAR; BAPTISTA, MAURICIO DA SILVA; ZAIDAN DAGLI, MARIA LUCIA; HERNANDEZ BLAZQUEZ, FRANCISCO JAVIER; et al. Effects of methylene blue-mediated photodynamic therapy on a mouse model of squamous cell carcinoma and normal skin. Photodiagnosis and Photodynamic Therapy, v. 23, p. 154-164, SEP 2018. (11/19752-7, 10/52675-3)