Evaluation of the effects of CTT1-loaded liquid crystalline system associated to photodynamic therapy in tumor growth using chorioallantoic membrane

• Grant number: 16/06337-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): May 31, 2016
• Effective date (End): August 30, 2016
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Marlus Chorilli
• Grantee: Giovana Maria Fioramonti Calixto Balian
• Supervisor: Maria de Fátima Monginho Baltazar
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Research place: Universidade do Minho (UMinho), Portugal
• Associated to the scholarship: 13/01565-1 - Mucoadhesive precursor systems of liquids crystals for buccal admnistration of antigelatinolytic peptide associated with photodynamic therapy in the treatment of oral cancer, BP.DR

Abstract

The cancer, disease characterized by the uncontrolled and exaggerated growth of cells, is considered a public health problem. Every year more than 12.5 million people worldwide are diagnosed with this disease, and 7.6 million die victims mainly of metastasis, which is a process of invasion and spreading of these cells to other organs. One strategy to reduce metastasis and to control the growth and spread of tumors is the administration of anti-angiogenic drugs, as the CTT1 peptide whose use has been shown to be a novel and effective alternative, since studies have shown that this peptide decreased tumor growth in mice. However, the clinical use of this peptide faces many challenges, mainly due to its physical and chemical characteristics and low oral bioavailability, which involves the administration by parenteral route. The use of this route of administration can result in poor patient compliance for the treatment, because it needs of repeated injections, since the CTT1 have reduced half life. Therefore, it becomes necessary to develop clinically safe and appropriate drug delivery systems to facilitate the use of this peptide, such as liquid crystalline systems (LCS) composed by surfactants that self-assemble in the presence of water to form liquid crystallines aggregates that can enhance the permeation of drugs, in addition to controlling its release. In addition, the LCS may enable the incorporation of CTT1 with the methylene blue (MB) photosensitizer, allowing the combination of photodynamic therapy (PDT) with the peptide antitumor therapy, which can enhance the treatment of cancer. In this context, this project aims to evaluate during the research internship at the University of Minho the action of this combination therapy on tumor growth using a Chick Chorioallantoic Membrane (CAM) as atumor model. CAM has a favorable immune system to insertion tumor cell lines and may give a tumor microvasculature in isolated environment, which allows a more controlled study CTT1 peptide action of SLC incorporated into or associated to PDT in angiogenesis and tumor growth. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VICTORELLI, FRANCESCA DAMIANI; DE OLIVEIRA CARDOSO, VALERIA MARIA; FERREIRA, NATALIA NORONHA; FIORAMONTI CALIXTO, GIOVANA MARIA; FONTANA, CARLA RAQUEL; BALTAZAR, FATIMA; DAFLON GREMIAO, MARIA PALMIRA; CHORILLI, MARLUS. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 153, p. 273-284, AUG 2020. (14/50928-2, 19/07245-5, 18/04546-1, 13/01565-1, 16/09671-3, 15/21412-0, 16/06337-5, 17/10016-2, 17/23357-2)

CALIXTO, GIOVANA MARIA FIORAMONTI; VICTORELLI, FRANCESCA DAMIANI; FRANZ-MONTAN, MICHELLE; BALTAZAR, FATIMA; CHORILLI, MARLUS. Innovative Mucoadhesive Precursor of Liquid Crystalline System Loading Anti-Gellatinolytic Peptide for Topical Treatment of Oral Cancer. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 17, n. 2, p. 253-262, FEB 2021. (14/50928-2, 19/07245-5, 16/06337-5)

VICTORELLI, FRANCESCA DAMIANI; DE OLIVEIRA CARDOSO, VALERIA MARIA; FERREIRA, NATALIA NORONHA; FIORAMONTI CALIXTO, GIOVANA MARIA; FONTANA, CARLA RAQUEL; BALTAZAR, FATIMA; DAFLON GREMIAO, MARIA PALMIRA; CHORILLI, MARLUS. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 153, p. 12-pg., 2020-08-01. (17/10016-2, 17/23357-2, 19/07245-5, 13/01565-1, 18/04546-1, 16/06337-5, 15/21412-0, 14/50928-2, 16/09671-3)