| Grant number: | 10/52167-8 |
| Support Opportunities: | Regular Research Grants |
| Start date: | April 01, 2011 |
| End date: | March 31, 2013 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Principal Investigator: | Luis Carlos de Souza Ferreira |
| Grantee: | Luis Carlos de Souza Ferreira |
| Host Institution: | Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
Abstract
The main objective of this project is the development of subunit vaccines administered via mucosal routes aiming the control of infections associated with strains of ETEC, a major etiological agent of infant diarrhea and travelers' diarrhea. The vaccine formulations to be tested will employ the purified CFA/I fimbriae and its subunit adhesive protein, CfaE. Vaccines to be tested will be added with a mucosal adjuvant, a nontoxic derivative of the heat-labile toxin (LT) also produced by ETEC strains. The vaccine formulations will be administered to DBA/2 mice by the oral and nasal routes and the presence of specific antibodies will be measured in serum and secretions (milk and fecal extracts). Protective efficacy of the induced antibodies will be determined by the in vitro neutralization of ETEC CFA/I+ adhesive properties. The work also provides the use of an animal model, based on newborns of the DBA2 lineage, as a correlate of protection afforded by vaccination against ETEC strains derived from human hosts. The results to be obtained should contribute to the development of new acellular vaccine formulations against diarrhea associated with ETEC infections for clinical and/or veterinarian use. (AU)
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