1. modulation of double-stranded RNA-Activated protein kinase in insulin sensitive...
| Grant number: | 11/03528-0 |
| Support type: | Regular Research Grants |
| Duration: | June 01, 2011 - May 31, 2013 |
| Field of knowledge: | Biological Sciences - Physiology |
| Principal Investigator: | Ronaldo de Carvalho Araújo |
| Grantee: | Ronaldo de Carvalho Araújo |
| Home Institution: | Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil |
Abstract
Obesity is a worldwide epidemy and it is related with increase of prevalence highlights the demographic, socio economic and epidemiological transition that led to a decrease in nutritional patterns and physical activity. This disease is associated with subclinical inflammation, characterized by abnormal production of proinflammatory cytokines. Various cells such as adipocytes and macrophages, are involved in this irregular pattern of cytokines which prevents proper intracellular signaling of insulin providing the state of insulin resistance and the onset of Diabetes type II. Is well established that physical exercise as a model measurable stress induction causes functional changes in the immune system. However, little is known about the exercise as a stimulator of macrophages and its effects on immune modulation of diseases related to obesity. From these data we aimed to assess the role of physical exercise on the inflammatory response of adipose tissue of mice subjected to high fat diet. To demonstrate this phenomenon, animals fed a control diet or high fat diet will be submitted for two swimming protocols: the first group will training for 15 minutes daily in an attempt not weight loss and a second will swim for 60 minutes a day where we expect a decrease in adiposity. Another group of animals fed a high fat diet will undergo a food restriction of 40% to compare with the group exercised for 60 minutes. Will be evaluated by flow cytometry the number of CD4 +, CD8 +, neutrophils and macrophages in inguinal adipose tissue. The tissue concentration and plasma adipokines 17 pro or anti-inflammatory will be evaluated using the test bioplex. It will be also assessed insulin resistance by the glucose tolerance test and insulin and blood lipid parameters before and after intervention. To estimate the role of leptin in adipose tissue inflammation and in response to exercise animals deficient for leptin (ob / ob) will be subject to the same procedures described above. (AU)