Obesity is a multifactorial disease, chronic and non-transmissible, defined by the accumulation of adipose tissue that is commonly associated with systemic inflammation. This state is linked to health damage and has increased its incidence worldwide. This disorder has been correlated as responsible for the appearance of various eye diseases and consequently damage to visual acuity. More specifically, cardiometabolic risk, due to the progression of the inflammatory profile, has been shown to be associated with lacrimal gland dysfunction, implicating in the development and aggravation of Cerato-Conjunctivitis Sicca or popularly known as dry eye disease. In more severe and extreme cases, the condition of obesity and inflammation can trigger the total loss of vision. In this scenario, in addition to its numerous metabolic functions, clusterin has been described as protective of the ocular surface by a mechanism of seal, which assists in cell survival. On the other hand, it is known that physical exercise can act as an important non-pharmacological tool in the fight against inflammation and obesity. However, no study was pereformed in order to investigate whether regular practice of different physical exercise protocols can modulate the tear composition and the clusterin signaling pathway in the retina and thus promote an improvement in visual acuity. Thus, the present study aims to elucidate the role of different chronic protocols of physical exercises (aerobic, strength and combined) in the levels of clusterin in the tear and to evaluate the metabolism of clusterin in the retina of obese mice. As preliminary results, through bioinformatics analyzes, we observed that there are the genes of clusterin and its receptor LRP1 / 2 in the eye and retina. Moreover, with the project already in progress, we observed that obese and insulin-resistant mice present increased gene expression of TNF-± and IL-1² and also elevated levels of these proinflammatory molecules in the retina. In addition, it was possible to observe that these animals presented reduction of clusterin levels also in the retina. After the conclusion of this study, it is expected to discover if the physical exercise is capable of promoting changes in the ocular system and if yes, unraveling through the metabolism of clusterin, this practice can improve visual acuity. (AU)
News published in Agência FAPESP Newsletter about the scholarship:
PEREIRA DE CAMPOS, THAIS DANTIS;
DA CRUZ RODRIGUES, KELLEN CRISTINA;
PEREIRA, RODRIGO MARTINS;
MORELLI, ANA PAULA;
DA ROCHA, ALISSON LUIZ;
CANCIGLIERI, RAPHAEL DOS SANTOS;
RAMOS DA SILVA, ADELINO SANCHEZ;
ROPELLE, EDUARDO ROCHETE;
PAULI, JOSE RODRIGO;
SIMABUCO, FERNANDO MOREIRA;
CINTRA, DENNYS ESPER;
DE MOURA, LEANDRO PEREIRA.
Short-Term Combined Exercise Improves Inflammatory Profile in the Retina of Obese Mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,
Web of Science Citations: 0.
(References retrieved automatically from State of São Paulo Research Institutions)