Enzymes in asymmetric synthesis: directed evolution of Rhodotorula glutinis and Pichia stipitis epoxide hydrolases

• Grant number: 11/06209-3
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2011
• End date: December 31, 2013
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luciana Gonzaga de Oliveira
• Grantee: Luciana Gonzaga de Oliveira
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Epoxide hydrolases are a group of hydrolitic enzymes free of co-factors. Such enzymes are widely spread in several organisms and act converting epoxides into the corresponding diols. Once this reaction could happen in a stereoselective fashion, epoxide hydrolases represent attractive asymmetric biocatalysts. Herein we propose the cloning and expression of Rhodotorula glutinis and Pichia stipitis epoxide hydrolases in Pichia pastoris, an efficient system for the expression of eukaryotic genes. After expression and chemical characterization of the enzyme catalytic activity we intend to exploit directed evolution approach combining appropriate methods for random gene mutagenesis/expression/screening or selection looking for enantioselective enzymes. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHAGAS, FERNANDA O.; RUZZINI, ANTONIO C.; BACHA, V, LARISSA; SAMBORSKYY, MARKYIAN; CONTI, RAPHAEL; PESSOTTI, RITA C.; DE OLIVEIRA, LUCIANA G.; CLARDY, JON; PUPO, MONICA T.. Genome Sequence of Streptomyces sp. Strain RTd22, an Endophyte of the Mexican Sunflower. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 4, JUL-AUG 2016. (13/07600-3, 08/09540-0, 13/50954-0, 09/17695-6, 08/00812-7, 11/06209-3, 11/12910-6, 11/11703-7)

CHAGAS, FERNANDA O.; RUZZINI, ANTONIO C.; BACHA, LARISSA, V; SAMBORSKYY, MARKYIAN; CONTI, RAPHAEL; PESSOTTI, RITA C.; DE OLIVEIRA, LUCIANA G.; CLARDY, JON; PUPO, MONICA T.. Genome Sequence of Streptomyces sp. Strain RTd22, an Endophyte of the Mexican Sunflower. GENOME ANNOUNCEMENTS, v. 4, n. 4, p. 2-pg., 2016-07-01. (13/07600-3, 13/50954-0, 11/06209-3, 08/09540-0, 11/12910-6, 11/11703-7, 09/17695-6, 08/00812-7)

DE OLIVEIRA, LUCIANA G.; TORMET GONZALEZ, GABRIELA D.; SAMBORSKY, MARKYIAN; MARCON, JOELMA; ARAUJO, WELINGTON L.; DE AZEVEDO, JOAO LUCIO. Genome Sequence of Streptomyces wadayamensis Strain A23, an Endophytic Actinobacterium from Citrus reticulata. GENOME ANNOUNCEMENTS, v. 2, n. 4, p. 2-pg., 2014-07-01. (11/06209-3)

TORMET-GONZALEZ, GABRIELA D.; WILSON, CAROLINA; DE OLIVEIRA, GABRIEL STEPHANI; DOS SANTOS, JADEMILSON CELESTINO; DE OLIVEIRA, LUCIANA G.; DIAS, MARCIO VINICIUS BERTACINE. An epoxide hydrolase from endophytic Streptomycess hows unique structural features and wide biocatalytic activity. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v. 76, p. 8-pg., 2020-09-01. (19/10564-5, 18/00351-1, 15/09188-8, 11/06209-3, 14/50249-8, 10/15971-3, 13/26242-0, 14/12727-5)

Filed patent(s) as a result of this research project