Evaluation of the influence of temperature and storage time in the detection and quantification of oral cavity bacteria samples by the DNA Checkerboard technique.

• Grant number: 10/17807-6
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2011
• End date: June 30, 2013
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Vinícius Pedrazzi
• Grantee: Vinícius Pedrazzi
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Paola Kirsten Miani ; Rubens Ferreira de Albuquerque Junior

Abstract

Molecular diagnostics methods have been widely used to detect and quantify bacterial species from oral cavity. The long-term storage of bacterial DNA samples may compromise the genetic material of the species interfering in the final results providing by these methods. The aim of this study is to evaluate the effect of temperature and storage time on the final quality of the DNA samples from oral cavity. Clinical samples of saliva and supra-gingival biofilm will be harvested from 10 healthy subjects and assessed by DNA Checkerboard hybridization technique. Samples will be tested immediately after taken (baseline-control) and after 2 weeks, 1 month, 6 months or 12 months of storage at room temperature, 4°C and -20°C. In addition to the detection and counting of the bacterial species, a correlation between the samples from the proposed protocols will be performed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO NASCIMENTO, CASSIO; DOS SANTOS, JANINE NAVARRO; PEDRAZZI, VINICIUS; PITA, MURILLO SUCENA; MONESI, NADIA; RIBEIRO, RICARDO FARIA; DE ALBUQUERQUE JUNIOR, RUBENS FERREIRA. Impact of temperature and time storage on the microbial detection of oral samples by Checkerboard DNA-DNA hybridization method. ARCHIVES OF ORAL BIOLOGY, v. 59, n. 1, p. 12-21, JAN 2014. (10/17807-6, 11/10008-3)