Training associated with N-acetylcysteine and modulation of purinergic receptors in the progression of experimental diabetic nephropathy

• Grant number: 11/12578-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2011
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Elisa Mieko Suemitsu Higa
• Grantee: Elisa Mieko Suemitsu Higa
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Cassia Marta de Toledo Bergamaschi ; Maria José da Silva Fernandes

Abstract

Diabetic nephropathy stands out as the main determinant of end-stage renal disease and oxidative stress (increase in reactive oxygen species - ROS) and endothelial dysfunction are some of its major aggravating factors. Recent studies in our Laboratory showed that the diabetic rats had reduced body weight, increased levels of creatinine and urea excretion and plasma TBARS as compared with respective controls; these effects were attenuated with exercises. N-acetylcysteine (NAC), an antioxidant, reduces ROS and attenuates the endothelial dysfunction; similar effects have been observed in training protocols. This study will evaluate the oxidative stress and activation of the purinergic receptors in uninephrectomized (UNx) rats, subjected to training and/or supplementation with NAC. Male Wistar rats with 8 weeks of age will be all UNx. Diabetes Mellitus (DM) will be induced with streptozotocin (60mg/kg, iv) in half of these animals, and the others will receive the vehicle (CTL). Half of both groups will be trained (CTL+EX and DM+EX) and the others will be considered sedentary (CTL+SE and DM+SE). NAC (600mg/L) will be given in drinking water in half of each group: CTL+SE+NAC, CTL+EX+NAC, DM+SE+NAC and DM+EX+NAC. The exercise training will consist of treadmill running, at 16m/min for 60min/day, 5 days/week for 8 weeks. Before and after the exercise period, blood samples and 24 hours urine will be collected. Data will be expressed as mean ±SEM, with one way ANOVA with Student-Newman-Keuls post-hoc analysis, with significance of P<0.05. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

THAMIRES DE OLIVEIRA FERNANDES; ADELSON MARÇAL RODRIGUES; GIOVANA RITA PUNARO; DEYSE YORGOS DE LIMA; ELISA MIEKO SUEMITSU HIGA. A interação do receptor P2X7 com o óxido nítrico medeia a apoptose em células mesangiais imortalizadas de camundongos expostas a níveis elevados de glicose. J. Bras. Nefrol., 2021-10-15. (11/12578-1)

THAMIRES DE OLIVEIRA FERNANDES; ADELSON MARÇAL RODRIGUES; GIOVANA RITA PUNARO; DEYSE YORGOS DE LIMA; ELISA MIEKO SUEMITSU HIGA. A interação do receptor P2X7 com o óxido nítrico medeia a apoptose em células mesangiais imortalizadas de camundongos expostas a níveis elevados de glicose. J. Bras. Nefrol., v. 44, n. 2, p. 147-154, 2021-10-15. (11/12578-1)

FERNANDES, THAMIRES DE OLIVEIRA; RODRIGUES, ADELSON MARCAL; PUNARO, GIOVANA RITA; DE LIMA, DEYSE YORGOS; SUEMITSU HIGA, ELISA MIEKO. P2X(7) receptor-nitric oxide interaction mediates apoptosis in mouse immortalized mesangial cells exposed to high glucose. J. Bras. Nefrol., v. 44, n. 2, p. 8-pg., 2022-01-01. (11/12578-1)